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A detection-based framework for the analysis of recycling in TIRF microscopy

  • A. Chessel
  • , B. Cinquin
  • , S. Bardin
  • , J. Boulanger
  • , J. Salamero
  • , C. Kervrann
  • Institut Curie
  • Centre national de la recherche scientifique
  • Synchrotron SOLEIL
  • Austrian Academy of Sciences
  • INRIA Institut National de Recherche en Informatique et en Automatique
  • UR341 Mathématiques et Informatique Appliquées

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Endocytosis/recycling and exocytosis aremechanisms conserved through evolution allowing cells to communicate with their external medium. In order to study these dynamic processes, the present work proposes a patch-based method for detecting recycling or exocytotic events at the Plasma membrane in fast TIRF microscopy combined with the computation of normalized temporal representations of those events. Evaluation, performed on TIRF sequences showing Transferrin receptor (TfR) recycling, validates a high detection rate fully compatible with an automatic data extraction and analysis of the plasma membrane recycling process.

Original languageEnglish
Title of host publication2010 7th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2010 - Proceedings
PublisherIEEE Computer Society
Pages1281-1284
Number of pages4
ISBN (Print)9781424441266
DOIs
Publication statusPublished - 1 Jan 2010
Event7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Rotterdam, Netherlands
Duration: 14 Apr 201017 Apr 2010

Publication series

Name2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings

Conference

Conference7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010
Country/TerritoryNetherlands
CityRotterdam
Period14/04/1017/04/10

Keywords

  • Endocytosis/recycling
  • Event detection
  • Patch-based
  • TIRFM
  • TfR

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