Advanced Design of Structural RNAs Using RNARedPrint

Yann Ponty, Stefan Hammer, Hua Ting Yao, Sebastian Will

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

RNA design addresses the need to build novel RNAs, e.g., for biotechnological applications in synthetic biology, equipped with desired functional properties. This chapter describes how to use the software RNARedPrint for the de novo rational design of RNA sequences adopting one or several desired secondary structures. Depending on the application, these structures could represent alternate configurations or kinetic pathways. The software makes such design convenient and sufficiently fast for practical routine, where it even overcomes notorious problems in the application of RNA design, e.g., it maintains realistic GC content.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-15
Number of pages15
DOIs
Publication statusPublished - 1 Jan 2021

Publication series

NameMethods in Molecular Biology
Volume2284
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Kinetic landscapes
  • RNA design
  • Riboswitches

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