Analysis of an activated ABI5 allele using a new selection method for transgenic Arabidopsis seeds

Sandra Bensmihen; Alexandra To; Guillaume Lambert; Thomas Kroj; Jérôme Giraudat; François Parcy

doi:10.1016/S0014-5793(04)00148-6

Analysis of an activated ABI5 allele using a new selection method for transgenic Arabidopsis seeds

Sandra Bensmihen
, Alexandra To
, Guillaume Lambert
, Thomas Kroj
, Jérôme Giraudat
, François Parcy

Research output: Contribution to journal › Article › peer-review

Abstract

The Arabidopsis abscisic acid (ABA) insensitive (ABI)5 transcription factor participates in the ABA-dependent induction of late embryogenesis abundant (LEA) genes in the final stages of seed development. We tested whether the VP16 transcriptional activation domain is sufficient to provide ABI5 with the ability to activate the AtEm LEA genes in vegetative tissues. We took advantage of a new transgenic seed selection assay based on green fluorescent protein (GFP) fluorescence and found that VP16-ABI5 triggered growth retardation and ABA-independent induction of AtEm1 in seedlings. These results indicate that ABI5 activation potential is a limiting step and might be a target for ABA signaling.

Original language	English
Pages (from-to)	127-131
Number of pages	5
Journal	FEBS Letters
Volume	561
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(04)00148-6
Publication status	Published - 12 Mar 2004
Externally published	Yes

Keywords

ABA, abscisic acid
ABI, abscisic acid insensitive
Abscisic acid
Abscisic acid insensitive 5
LEA, late embryogenesis abundant
Late embryogenesis abundant gene regulation
Transgenic seed selection
VP16-AD, VP16 activation domain

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10.1016/S0014-5793(04)00148-6

Cite this

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title = "Analysis of an activated ABI5 allele using a new selection method for transgenic Arabidopsis seeds",

abstract = "The Arabidopsis abscisic acid (ABA) insensitive (ABI)5 transcription factor participates in the ABA-dependent induction of late embryogenesis abundant (LEA) genes in the final stages of seed development. We tested whether the VP16 transcriptional activation domain is sufficient to provide ABI5 with the ability to activate the AtEm LEA genes in vegetative tissues. We took advantage of a new transgenic seed selection assay based on green fluorescent protein (GFP) fluorescence and found that VP16-ABI5 triggered growth retardation and ABA-independent induction of AtEm1 in seedlings. These results indicate that ABI5 activation potential is a limiting step and might be a target for ABA signaling.",

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author = "Sandra Bensmihen and Alexandra To and Guillaume Lambert and Thomas Kroj and J{\'e}r{\^o}me Giraudat and Fran{\c c}ois Parcy",

year = "2004",

month = mar,

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doi = "10.1016/S0014-5793(04)00148-6",

language = "English",

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journal = "FEBS Letters",

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T1 - Analysis of an activated ABI5 allele using a new selection method for transgenic Arabidopsis seeds

AU - Bensmihen, Sandra

AU - To, Alexandra

AU - Lambert, Guillaume

AU - Kroj, Thomas

AU - Giraudat, Jérôme

AU - Parcy, François

PY - 2004/3/12

Y1 - 2004/3/12

N2 - The Arabidopsis abscisic acid (ABA) insensitive (ABI)5 transcription factor participates in the ABA-dependent induction of late embryogenesis abundant (LEA) genes in the final stages of seed development. We tested whether the VP16 transcriptional activation domain is sufficient to provide ABI5 with the ability to activate the AtEm LEA genes in vegetative tissues. We took advantage of a new transgenic seed selection assay based on green fluorescent protein (GFP) fluorescence and found that VP16-ABI5 triggered growth retardation and ABA-independent induction of AtEm1 in seedlings. These results indicate that ABI5 activation potential is a limiting step and might be a target for ABA signaling.

AB - The Arabidopsis abscisic acid (ABA) insensitive (ABI)5 transcription factor participates in the ABA-dependent induction of late embryogenesis abundant (LEA) genes in the final stages of seed development. We tested whether the VP16 transcriptional activation domain is sufficient to provide ABI5 with the ability to activate the AtEm LEA genes in vegetative tissues. We took advantage of a new transgenic seed selection assay based on green fluorescent protein (GFP) fluorescence and found that VP16-ABI5 triggered growth retardation and ABA-independent induction of AtEm1 in seedlings. These results indicate that ABI5 activation potential is a limiting step and might be a target for ABA signaling.

KW - ABA, abscisic acid

KW - ABI, abscisic acid insensitive

KW - Abscisic acid

KW - Abscisic acid insensitive 5

KW - LEA, late embryogenesis abundant

KW - Late embryogenesis abundant gene regulation

KW - Transgenic seed selection

KW - VP16-AD, VP16 activation domain

UR - https://www.scopus.com/pages/publications/1542328221

U2 - 10.1016/S0014-5793(04)00148-6

DO - 10.1016/S0014-5793(04)00148-6

M3 - Article

C2 - 15013763

AN - SCOPUS:1542328221

SN - 0014-5793

VL - 561

SP - 127

EP - 131

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Analysis of an activated ABI5 allele using a new selection method for transgenic Arabidopsis seeds

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Keywords

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