Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation

Anne Garlonn Desbrosses-Fonrouge; Katrin Voigt; Astrid Schröder; Stéphanie Arrivault; Sébastien Thomine; Ute Krämer

doi:10.1016/j.febslet.2005.06.046

Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation

Anne Garlonn Desbrosses-Fonrouge
, Katrin Voigt
, Astrid Schröder
, Stéphanie Arrivault
, Sébastien Thomine
, Ute Krämer

Research output: Contribution to journal › Article › peer-review

Abstract

The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1_D94A mutant or water-injected oocytes. An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.

Original language	English
Pages (from-to)	4165-4174
Number of pages	10
Journal	FEBS Letters
Volume	579
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2005.06.046
Publication status	Published - 1 Aug 2005
Externally published	Yes

Keywords

Cation efflux
Hyperaccumulation
Intron-spliced hairpin
Metal tolerance
ZAT
Zinc

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10.1016/j.febslet.2005.06.046

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title = "Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation",

abstract = "The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1D94A mutant or water-injected oocytes. An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.",

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year = "2005",

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doi = "10.1016/j.febslet.2005.06.046",

language = "English",

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T1 - Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation

AU - Desbrosses-Fonrouge, Anne Garlonn

AU - Voigt, Katrin

AU - Schröder, Astrid

AU - Arrivault, Stéphanie

AU - Thomine, Sébastien

AU - Krämer, Ute

PY - 2005/8/1

Y1 - 2005/8/1

N2 - The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1D94A mutant or water-injected oocytes. An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.

AB - The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1D94A mutant or water-injected oocytes. An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.

KW - Cation efflux

KW - Hyperaccumulation

KW - Intron-spliced hairpin

KW - Metal tolerance

KW - ZAT

KW - Zinc

UR - https://www.scopus.com/pages/publications/22544439037

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DO - 10.1016/j.febslet.2005.06.046

M3 - Article

C2 - 16038907

AN - SCOPUS:22544439037

SN - 0014-5793

VL - 579

SP - 4165

EP - 4174

JO - FEBS Letters

JF - FEBS Letters

IS - 19

ER -

Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation

Abstract

Keywords

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