Assembly of chemically modified G-rich sequences into tetramolecular DNA G-quadruplexes and higher order structures

Jun Zhou; Frédéric Rosu; Samir Amrane; Dursun Nizam Korkut; Valérie Gabelica; Jean Louis Mergny

doi:10.1016/j.ymeth.2014.01.004

Assembly of chemically modified G-rich sequences into tetramolecular DNA G-quadruplexes and higher order structures

Jun Zhou
, Frédéric Rosu
, Samir Amrane
, Dursun Nizam Korkut
, Valérie Gabelica
, Jean Louis Mergny

Univ. Bordeaux
INSERM, U869, IECB

Research output: Contribution to journal › Article › peer-review

Abstract

In this review, we introduce the biophysical and biochemical methods currently used to investigate the structures and stabilities of tetramolecular DNA G-quadruplexes containing chemical modifications. We hope this paper will guide others as they perform similar experiments leading to more information about the effects of chemical modifications on G-quadruplex formation. The structures of tetramolecular quadruplexes and some higher order structures based on tetramolecular quadruplexes are also described.

Original language	English
Pages (from-to)	159-168
Number of pages	10
Journal	Methods
Volume	67
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2014.01.004
Publication status	Published - 15 May 2014
Externally published	Yes

Keywords

CD
Chemical modification
G-quadruplex
Mass spectrometry
NMR
UV

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10.1016/j.ymeth.2014.01.004

Cite this

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title = "Assembly of chemically modified G-rich sequences into tetramolecular DNA G-quadruplexes and higher order structures",

abstract = "In this review, we introduce the biophysical and biochemical methods currently used to investigate the structures and stabilities of tetramolecular DNA G-quadruplexes containing chemical modifications. We hope this paper will guide others as they perform similar experiments leading to more information about the effects of chemical modifications on G-quadruplex formation. The structures of tetramolecular quadruplexes and some higher order structures based on tetramolecular quadruplexes are also described.",

keywords = "CD, Chemical modification, G-quadruplex, Mass spectrometry, NMR, UV",

author = "Jun Zhou and Fr{\'e}d{\'e}ric Rosu and Samir Amrane and Korkut, \{Dursun Nizam\} and Val{\'e}rie Gabelica and Mergny, \{Jean Louis\}",

note = "Funding Information: Jun Zhou is the recipient of a Marie Curie International Incoming Fellowship ; S. Amrane benefits from ANRS funding . This work was supported by grants from ANR (G4Toolbox, F-DNA and Oligoswitch) Nanobiotech (Vibbnano; ANR -10-NANO-04) and Conseil R{\'e}gional d{\textquoteright}Aquitaine (“Chaire d{\textquoteright}accueil”) to J.L. Mergny. V. Gabelica is an ATIP/Avenir fellow.",

year = "2014",

month = may,

day = "15",

doi = "10.1016/j.ymeth.2014.01.004",

language = "English",

volume = "67",

pages = "159--168",

journal = "Methods",

issn = "1046-2023",

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TY - JOUR

T1 - Assembly of chemically modified G-rich sequences into tetramolecular DNA G-quadruplexes and higher order structures

AU - Zhou, Jun

AU - Rosu, Frédéric

AU - Amrane, Samir

AU - Korkut, Dursun Nizam

AU - Gabelica, Valérie

AU - Mergny, Jean Louis

N1 - Funding Information: Jun Zhou is the recipient of a Marie Curie International Incoming Fellowship ; S. Amrane benefits from ANRS funding . This work was supported by grants from ANR (G4Toolbox, F-DNA and Oligoswitch) Nanobiotech (Vibbnano; ANR -10-NANO-04) and Conseil Régional d’Aquitaine (“Chaire d’accueil”) to J.L. Mergny. V. Gabelica is an ATIP/Avenir fellow.

PY - 2014/5/15

Y1 - 2014/5/15

N2 - In this review, we introduce the biophysical and biochemical methods currently used to investigate the structures and stabilities of tetramolecular DNA G-quadruplexes containing chemical modifications. We hope this paper will guide others as they perform similar experiments leading to more information about the effects of chemical modifications on G-quadruplex formation. The structures of tetramolecular quadruplexes and some higher order structures based on tetramolecular quadruplexes are also described.

AB - In this review, we introduce the biophysical and biochemical methods currently used to investigate the structures and stabilities of tetramolecular DNA G-quadruplexes containing chemical modifications. We hope this paper will guide others as they perform similar experiments leading to more information about the effects of chemical modifications on G-quadruplex formation. The structures of tetramolecular quadruplexes and some higher order structures based on tetramolecular quadruplexes are also described.

KW - CD

KW - Chemical modification

KW - G-quadruplex

KW - Mass spectrometry

KW - NMR

KW - UV

U2 - 10.1016/j.ymeth.2014.01.004

DO - 10.1016/j.ymeth.2014.01.004

M3 - Article

C2 - 24434505

AN - SCOPUS:84899737914

SN - 1046-2023

VL - 67

SP - 159

EP - 168

JO - Methods

JF - Methods

IS - 2

ER -

Assembly of chemically modified G-rich sequences into tetramolecular DNA G-quadruplexes and higher order structures

Abstract

Keywords

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