Bulges in left-handed G-quadruplexes

Poulomi Das; Khac Huy Ngo; Fernaldo Richtia Winnerdy; Arijit Maity; Blaž Bakalar; Yves Mechulam; Emmanuelle Schmitt; Anh Tuân Phan

doi:10.1093/nar/gkaa1259

Bulges in left-handed G-quadruplexes

Poulomi Das
, Khac Huy Ngo
, Fernaldo Richtia Winnerdy
, Arijit Maity
, Blaž Bakalar
, Yves Mechulam
, Emmanuelle Schmitt
, Anh Tuân Phan

School of Physical and Mathematical Sciences
NTU Institute of Structural Biology

Research output: Contribution to journal › Article › peer-review

Abstract

G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.

Original language	English
Pages (from-to)	1724-1736
Number of pages	13
Journal	Nucleic Acids Research
Volume	49
Issue number	3
DOIs	https://doi.org/10.1093/nar/gkaa1259
Publication status	Published - 22 Feb 2021

Access to Document

10.1093/nar/gkaa1259

Cite this

@article{b63aaceed10a431ababc8daa0f12d9dd,

title = "Bulges in left-handed G-quadruplexes",

abstract = "G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.",

author = "Poulomi Das and Ngo, \{Khac Huy\} and Winnerdy, \{Fernaldo Richtia\} and Arijit Maity and Bla{\v z} Bakalar and Yves Mechulam and Emmanuelle Schmitt and Phan, \{Anh Tu{\^a}n\}",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.",

year = "2021",

month = feb,

day = "22",

doi = "10.1093/nar/gkaa1259",

language = "English",

volume = "49",

pages = "1724--1736",

journal = "Nucleic Acids Research",

issn = "0305-1048",

number = "3",

}

TY - JOUR

T1 - Bulges in left-handed G-quadruplexes

AU - Das, Poulomi

AU - Ngo, Khac Huy

AU - Winnerdy, Fernaldo Richtia

AU - Maity, Arijit

AU - Bakalar, Blaž

AU - Mechulam, Yves

AU - Schmitt, Emmanuelle

AU - Phan, Anh Tuân

PY - 2021/2/22

Y1 - 2021/2/22

N2 - G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.

AB - G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.

U2 - 10.1093/nar/gkaa1259

DO - 10.1093/nar/gkaa1259

M3 - Article

C2 - 33503265

AN - SCOPUS:85102216172

SN - 0305-1048

VL - 49

SP - 1724

EP - 1736

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 3

ER -

Bulges in left-handed G-quadruplexes

Abstract

Access to Document

Fingerprint

Cite this