Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone

Gelsomina De Stasio; B. Gilbert; L. Perfetti; R. Hansen; D. Mercanti; M. T. Ciotti; R. Andres; V. E. White; P. Perfetti; G. Margaritondo

doi:10.1006/abio.1998.2901

Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone

Gelsomina De Stasio
, B. Gilbert
, L. Perfetti
, R. Hansen
, D. Mercanti
, M. T. Ciotti
, R. Andres
, V. E. White
, P. Perfetti
, G. Margaritondo

Research output: Contribution to journal › Article › peer-review

Abstract

We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability.

Original language	English
Pages (from-to)	174-180
Number of pages	7
Journal	Analytical Biochemistry
Volume	266
Issue number	2
DOIs	https://doi.org/10.1006/abio.1998.2901
Publication status	Published - 15 Jan 1999
Externally published	Yes

Keywords

Ashing
Incineration
MEPHISTO
Microchemical analysis
Synchrotron spectromicroscopy
Trace element

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10.1006/abio.1998.2901

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title = "Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone",

abstract = "We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability.",

keywords = "Ashing, Incineration, MEPHISTO, Microchemical analysis, Synchrotron spectromicroscopy, Trace element",

author = "\{De Stasio\}, Gelsomina and B. Gilbert and L. Perfetti and R. Hansen and D. Mercanti and Ciotti, \{M. T.\} and R. Andres and White, \{V. E.\} and P. Perfetti and G. Margaritondo",

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doi = "10.1006/abio.1998.2901",

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T2 - A new approach based on ultraviolet/ozone

AU - De Stasio, Gelsomina

AU - Gilbert, B.

AU - Perfetti, L.

AU - Hansen, R.

AU - Mercanti, D.

AU - Ciotti, M. T.

AU - Andres, R.

AU - White, V. E.

AU - Perfetti, P.

AU - Margaritondo, G.

PY - 1999/1/15

Y1 - 1999/1/15

N2 - We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability.

AB - We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability.

KW - Ashing

KW - Incineration

KW - MEPHISTO

KW - Microchemical analysis

KW - Synchrotron spectromicroscopy

KW - Trace element

U2 - 10.1006/abio.1998.2901

DO - 10.1006/abio.1998.2901

M3 - Article

C2 - 9888973

AN - SCOPUS:0033555536

SN - 0003-2697

VL - 266

SP - 174

EP - 180

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone

Abstract

Keywords

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