Chiral chromophores for second harmonic microscopy

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The use of chiral harmonophores in second harmonic generation (SHG) microscopy of lipid bila yers should enable one to obtain a signal even when the distribution of the chromophores is centrosymmetric. In order to determine optimal chiral molecules, we performed polarization-resolved second harmonic re ection experiments. We found that chirality must arise from an excitonic coupling rather than from an asymmetric center. We selectively labeled giant unilamellar lipid vesicles and cell membranes with such a molecule, namely an acridine substituted Tröger's base, as demonstrated by two-photon-excited uorescence microscopy. We performed preliminary SHG microscopy experiments, but the poor efficiency of the current form of our molecule does not allow us to demonstrate chirality effects.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2003
PublisherOptica Publishing Group (formerly OSA)
Pages121-128
Number of pages8
ISBN (Electronic)0819450111
Publication statusPublished - 1 Jan 2003
EventEuropean Conference on Biomedical Optics, ECBO 2003 - Munich, Germany
Duration: 22 Jun 2003 → …

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO 2003
Country/TerritoryGermany
CityMunich
Period22/06/03 → …

Keywords

  • Nonlinear microscopy
  • chirality
  • giant unilamellar vesicles
  • lipid membranes
  • second harmonic generation

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