Chiral chromophores for second harmonic microscopy

M. C. Schanne-Klein; T. Boulesteix; E. Beaurepaire; M. P. Sauviat

doi:10.1117/12.499754

Chiral chromophores for second harmonic microscopy

M. C. Schanne-Klein, T. Boulesteix, E. Beaurepaire, M. P. Sauviat

Institut Polytechnique de Paris

Research output: Contribution to journal › Conference article › peer-review

Abstract

The use of chiral harmonophores in second harmonic generation (SHG) microscopy of lipid bilayers should enable one to obtain a signal even when the distribution of the chromophores is centrosymmetric. In order to determine optimal chiral molecules, we performed polarization-resolved second harmonic reflection experiments. We found that chirality must arise from an excitonic coupling rather than from an asymmetric center. We selectively labeled giant unilamellar lipid vesicles and cell membranes with such a molecule, namely an acridine substituted Tröger's base, as demonstrated by two-photon-excited fluorescence microscopy. We performed preliminary SHG microscopy experiments, but the poor efficiency of the current form of our molecule does not allow us to demonstrate chirality effects.

Original language	English
Pages (from-to)	121-128
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5139
DOIs	https://doi.org/10.1117/12.499754
Publication status	Published - 1 Jan 2003
Event	Confocal, Multiphoton, and Nonlinear Microscopic Imaging - Munich, Germany Duration: 22 Jun 2003 → 23 Jun 2003

Keywords

Chirality
Giant unilamellar vesicles
Lipid membranes
Nonlinear microscopy
Second harmonic generation

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10.1117/12.499754

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title = "Chiral chromophores for second harmonic microscopy",

abstract = "The use of chiral harmonophores in second harmonic generation (SHG) microscopy of lipid bilayers should enable one to obtain a signal even when the distribution of the chromophores is centrosymmetric. In order to determine optimal chiral molecules, we performed polarization-resolved second harmonic reflection experiments. We found that chirality must arise from an excitonic coupling rather than from an asymmetric center. We selectively labeled giant unilamellar lipid vesicles and cell membranes with such a molecule, namely an acridine substituted Tr{\"o}ger's base, as demonstrated by two-photon-excited fluorescence microscopy. We performed preliminary SHG microscopy experiments, but the poor efficiency of the current form of our molecule does not allow us to demonstrate chirality effects.",

keywords = "Chirality, Giant unilamellar vesicles, Lipid membranes, Nonlinear microscopy, Second harmonic generation",

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TY - JOUR

T1 - Chiral chromophores for second harmonic microscopy

AU - Schanne-Klein, M. C.

AU - Boulesteix, T.

AU - Beaurepaire, E.

AU - Sauviat, M. P.

PY - 2003/1/1

Y1 - 2003/1/1

N2 - The use of chiral harmonophores in second harmonic generation (SHG) microscopy of lipid bilayers should enable one to obtain a signal even when the distribution of the chromophores is centrosymmetric. In order to determine optimal chiral molecules, we performed polarization-resolved second harmonic reflection experiments. We found that chirality must arise from an excitonic coupling rather than from an asymmetric center. We selectively labeled giant unilamellar lipid vesicles and cell membranes with such a molecule, namely an acridine substituted Tröger's base, as demonstrated by two-photon-excited fluorescence microscopy. We performed preliminary SHG microscopy experiments, but the poor efficiency of the current form of our molecule does not allow us to demonstrate chirality effects.

AB - The use of chiral harmonophores in second harmonic generation (SHG) microscopy of lipid bilayers should enable one to obtain a signal even when the distribution of the chromophores is centrosymmetric. In order to determine optimal chiral molecules, we performed polarization-resolved second harmonic reflection experiments. We found that chirality must arise from an excitonic coupling rather than from an asymmetric center. We selectively labeled giant unilamellar lipid vesicles and cell membranes with such a molecule, namely an acridine substituted Tröger's base, as demonstrated by two-photon-excited fluorescence microscopy. We performed preliminary SHG microscopy experiments, but the poor efficiency of the current form of our molecule does not allow us to demonstrate chirality effects.

KW - Chirality

KW - Giant unilamellar vesicles

KW - Lipid membranes

KW - Nonlinear microscopy

KW - Second harmonic generation

U2 - 10.1117/12.499754

DO - 10.1117/12.499754

M3 - Conference article

AN - SCOPUS:1342268104

SN - 0277-786X

VL - 5139

SP - 121

EP - 128

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Confocal, Multiphoton, and Nonlinear Microscopic Imaging

Y2 - 22 June 2003 through 23 June 2003

ER -

Chiral chromophores for second harmonic microscopy

Abstract

Keywords

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