Color TSFG microscopy of red blood cells and oxygenation

Júlia Ferrer Ortas; Pierre Mahou; Sophie Escot; Chiara Stringari; Nicolas B. David; Laure Bally-Cuif; Nicolas Dray; Michel Négrerie; Willy Supatto; Emmanuel Beaurepaire

doi:10.1117/12.2670229

Color TSFG microscopy of red blood cells and oxygenation

Júlia Ferrer Ortas
, Pierre Mahou
, Sophie Escot
, Chiara Stringari
, Nicolas B. David
, Laure Bally-Cuif
, Nicolas Dray
, Michel Négrerie
, Willy Supatto
, Emmanuel Beaurepaire

Research output: Contribution to conference › Paper › peer-review

Abstract

We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.

Original language	English
DOIs	https://doi.org/10.1117/12.2670229
Publication status	Published - 1 Jan 2023
Event	2023 European Conference on Biomedical Optics, ECBO 2023 - Munich, Germany Duration: 25 Jun 2023 → 29 Jun 2023

Conference

Conference	2023 European Conference on Biomedical Optics, ECBO 2023
Country/Territory	Germany
City	Munich
Period	25/06/23 → 29/06/23

Access to Document

10.1117/12.2670229

Cite this

@conference{1485dcd23099439bacf6582f3e9b6fc1,

title = "Color TSFG microscopy of red blood cells and oxygenation",

abstract = "We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.",

author = "Ortas, \{J{\'u}lia Ferrer\} and Pierre Mahou and Sophie Escot and Chiara Stringari and David, \{Nicolas B.\} and Laure Bally-Cuif and Nicolas Dray and Michel N{\'e}grerie and Willy Supatto and Emmanuel Beaurepaire",

note = "Publisher Copyright: {\textcopyright} 2023 SPIE.; 2023 European Conference on Biomedical Optics, ECBO 2023 ; Conference date: 25-06-2023 Through 29-06-2023",

year = "2023",

month = jan,

day = "1",

doi = "10.1117/12.2670229",

language = "English",

}

TY - CONF

T1 - Color TSFG microscopy of red blood cells and oxygenation

AU - Ortas, Júlia Ferrer

AU - Mahou, Pierre

AU - Escot, Sophie

AU - Stringari, Chiara

AU - David, Nicolas B.

AU - Bally-Cuif, Laure

AU - Dray, Nicolas

AU - Négrerie, Michel

AU - Supatto, Willy

AU - Beaurepaire, Emmanuel

PY - 2023/1/1

Y1 - 2023/1/1

N2 - We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.

AB - We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.

U2 - 10.1117/12.2670229

DO - 10.1117/12.2670229

M3 - Paper

AN - SCOPUS:85214820070

T2 - 2023 European Conference on Biomedical Optics, ECBO 2023

Y2 - 25 June 2023 through 29 June 2023

ER -

Color TSFG microscopy of red blood cells and oxygenation

Abstract

Conference

Access to Document

Fingerprint

Cite this