TY - GEN
T1 - Correction accuracy in image-based adaptive optics for nonlinear microscopy
AU - Facomprez, Aurélie
AU - Beaurepaire, Emmanuel
AU - Débarre, Delphine
PY - 2012/4/16
Y1 - 2012/4/16
N2 - We investigate the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. In this paper we focus on the case of two-photon excited fluorescence. Using analytical, numerical and experimental results, we show that using a suitable number of measurements, accurate correction can be achieved for up to 2 rad rms initial aberrations even without optimisation of the correction modes. We demonstrate that this correction can be achieved using low light levels to minimise photobleaching and toxicity, and we provide examples of such optimised correction.
AB - We investigate the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. In this paper we focus on the case of two-photon excited fluorescence. Using analytical, numerical and experimental results, we show that using a suitable number of measurements, accurate correction can be achieved for up to 2 rad rms initial aberrations even without optimisation of the correction modes. We demonstrate that this correction can be achieved using low light levels to minimise photobleaching and toxicity, and we provide examples of such optimised correction.
KW - Adaptive optics
KW - aberration correction
KW - multiphoton microscopy
KW - nonlinear microscopy
U2 - 10.1117/12.908647
DO - 10.1117/12.908647
M3 - Conference contribution
AN - SCOPUS:84859579678
SN - 9780819488701
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Three-Dimensional and Multidimensional Microscopy
T2 - Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Y2 - 24 January 2012 through 26 January 2012
ER -