Cortical contractility triggers a stochastic switch to fast amoeboid cell motility

Verena Ruprecht; Stefan Wieser; Andrew Callan-Jones; Michael Smutny; Hitoshi Morita; Keisuke Sako; Vanessa Barone; Monika Ritsch-Marte; Michael Sixt; Raphaël Voituriez; Carl Philipp Heisenberg

doi:10.1016/j.cell.2015.01.008

Cortical contractility triggers a stochastic switch to fast amoeboid cell motility

Verena Ruprecht, Stefan Wieser, Andrew Callan-Jones, Michael Smutny, Hitoshi Morita, Keisuke Sako, Vanessa Barone, Monika Ritsch-Marte, Michael Sixt, Raphaël Voituriez, Carl Philipp Heisenberg

Research output: Contribution to journal › Article › peer-review

Abstract

3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.

Original language	English
Pages (from-to)	673-685
Number of pages	13
Journal	Cell
Volume	160
Issue number	4
DOIs	https://doi.org/10.1016/j.cell.2015.01.008
Publication status	Published - 12 Feb 2015
Externally published	Yes

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10.1016/j.cell.2015.01.008

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title = "Cortical contractility triggers a stochastic switch to fast amoeboid cell motility",

abstract = "3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.",

author = "Verena Ruprecht and Stefan Wieser and Andrew Callan-Jones and Michael Smutny and Hitoshi Morita and Keisuke Sako and Vanessa Barone and Monika Ritsch-Marte and Michael Sixt and Rapha{\"e}l Voituriez and Heisenberg, \{Carl Philipp\}",

note = "Publisher Copyright: {\textcopyright} 2015 The Authors.",

year = "2015",

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doi = "10.1016/j.cell.2015.01.008",

language = "English",

volume = "160",

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journal = "Cell",

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TY - JOUR

T1 - Cortical contractility triggers a stochastic switch to fast amoeboid cell motility

AU - Ruprecht, Verena

AU - Wieser, Stefan

AU - Callan-Jones, Andrew

AU - Smutny, Michael

AU - Morita, Hitoshi

AU - Sako, Keisuke

AU - Barone, Vanessa

AU - Ritsch-Marte, Monika

AU - Sixt, Michael

AU - Voituriez, Raphaël

AU - Heisenberg, Carl Philipp

PY - 2015/2/12

Y1 - 2015/2/12

N2 - 3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.

AB - 3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.

U2 - 10.1016/j.cell.2015.01.008

DO - 10.1016/j.cell.2015.01.008

M3 - Article

C2 - 25679761

AN - SCOPUS:84922702293

SN - 0092-8674

VL - 160

SP - 673

EP - 685

JO - Cell

JF - Cell

IS - 4

ER -

Cortical contractility triggers a stochastic switch to fast amoeboid cell motility

Abstract

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