Skip to main navigation Skip to search Skip to main content

Crystallization of macromolecular complexes: Combinatorial complex crystallization

  • Département d'Ingénierie Et d'Etudes Des Protéines (DIEP)
  • CEA/UVSQ/CNRS

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The structure of the Fab-staphylococcus aureus protein A (SpA) and Fab-peptostreptococcus magnus protein L (PpL) complexes was studied which was applied to a few ternary complexes. The use of Ig-biding proteins as a way of extending the method from HIV gp120 to all proteins for which there are monoclonal antibodies was also proposed. It was found that even with relatively unpurified samples, it was possible to discover crystallization. The results showed that Ig-binding proteins can positively influence the crystallization of Fab-antigen complexes.

Original languageEnglish
Pages (from-to)573-579
Number of pages7
JournalJournal of Crystal Growth
Volume232
Issue number1-4
DOIs
Publication statusPublished - 1 Nov 2001
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • A1. biocrystallization
  • A1. screening
  • B1. antibodies
  • B1. biological macromolecules

Fingerprint

Dive into the research topics of 'Crystallization of macromolecular complexes: Combinatorial complex crystallization'. Together they form a unique fingerprint.

Cite this