Deep and fast live imaging with two-photon scanned light-sheet microscopy

Thai V. Truong; Willy Supatto; David S. Koos; John M. Choi; Scott E. Fraser

doi:10.1038/nmeth.1652

Deep and fast live imaging with two-photon scanned light-sheet microscopy

Thai V. Truong
, Willy Supatto
, David S. Koos
, John M. Choi
, Scott E. Fraser

Beckman Institute

Research output: Contribution to journal › Article › peer-review

Abstract

We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.

Original language	English
Pages (from-to)	757-762
Number of pages	6
Journal	Nature Methods
Volume	8
Issue number	9
DOIs	https://doi.org/10.1038/nmeth.1652
Publication status	Published - 1 Jan 2011

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10.1038/nmeth.1652

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title = "Deep and fast live imaging with two-photon scanned light-sheet microscopy",

abstract = "We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.",

author = "Truong, \{Thai V.\} and Willy Supatto and Koos, \{David S.\} and Choi, \{John M.\} and Fraser, \{Scott E.\}",

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AU - Supatto, Willy

AU - Koos, David S.

AU - Choi, John M.

AU - Fraser, Scott E.

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AB - We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.

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Deep and fast live imaging with two-photon scanned light-sheet microscopy

Abstract

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