Design superiority of palindromic DNA sites for site-specific recognition of proteins: Tests using protein stitchery

Changmoon Park; Judy L. Campbell; William A. Goddard

doi:10.1073/pnas.90.11.4892

Design superiority of palindromic DNA sites for site-specific recognition of proteins: Tests using protein stitchery

Changmoon Park
, Judy L. Campbell
, William A. Goddard

Materials and Molecular Simulation Center
Beckman Institute
Division of Chemistry and Chemical Engineering
Division of Biology and Biological Engineering

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Using protein stitchery with appropriate attachment of cysteines linking to either C or N termini of the basic region of the v-Jun leucine zipper gene-regulatory protein, we constructed three dimers - pCC, pCN, and pNN. All three bind specifically to the appropriately rearranged DNA recognition sites for v-Jun: ATGAcgTCAT, ATGAcgATGA, and TCATcgTCAT, respectively (K_d, ≈4 nM at 4°C). Results of DNase I footprinting provide strong support for bent recognition helices in leucine zipper protein-DNA complexes. Comparison of the results for pCC and pNN with those for pCN shows the design superiority of palindromic sequences for protein recognition.

Original language	English
Pages (from-to)	4892-4896
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	11
DOIs	https://doi.org/10.1073/pnas.90.11.4892
Publication status	Published - 1 Jun 1993
Externally published	Yes

Access to Document

10.1073/pnas.90.11.4892

Cite this

@article{3df95793c95c4440b256177f85439b4a,

title = "Design superiority of palindromic DNA sites for site-specific recognition of proteins: Tests using protein stitchery",

abstract = "Using protein stitchery with appropriate attachment of cysteines linking to either C or N termini of the basic region of the v-Jun leucine zipper gene-regulatory protein, we constructed three dimers - pCC, pCN, and pNN. All three bind specifically to the appropriately rearranged DNA recognition sites for v-Jun: ATGAcgTCAT, ATGAcgATGA, and TCATcgTCAT, respectively (Kd, ≈4 nM at 4°C). Results of DNase I footprinting provide strong support for bent recognition helices in leucine zipper protein-DNA complexes. Comparison of the results for pCC and pNN with those for pCN shows the design superiority of palindromic sequences for protein recognition.",

author = "Changmoon Park and Campbell, \{Judy L.\} and Goddard, \{William A.\}",

year = "1993",

month = jun,

day = "1",

doi = "10.1073/pnas.90.11.4892",

language = "English",

volume = "90",

pages = "4892--4896",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "11",

}

Design superiority of palindromic DNA sites for site-specific recognition of proteins: Tests using protein stitchery. / Park, Changmoon; Campbell, Judy L.; Goddard, William A.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 11, 01.06.1993, p. 4892-4896.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Design superiority of palindromic DNA sites for site-specific recognition of proteins

T2 - Tests using protein stitchery

AU - Park, Changmoon

AU - Campbell, Judy L.

AU - Goddard, William A.

PY - 1993/6/1

Y1 - 1993/6/1

N2 - Using protein stitchery with appropriate attachment of cysteines linking to either C or N termini of the basic region of the v-Jun leucine zipper gene-regulatory protein, we constructed three dimers - pCC, pCN, and pNN. All three bind specifically to the appropriately rearranged DNA recognition sites for v-Jun: ATGAcgTCAT, ATGAcgATGA, and TCATcgTCAT, respectively (Kd, ≈4 nM at 4°C). Results of DNase I footprinting provide strong support for bent recognition helices in leucine zipper protein-DNA complexes. Comparison of the results for pCC and pNN with those for pCN shows the design superiority of palindromic sequences for protein recognition.

AB - Using protein stitchery with appropriate attachment of cysteines linking to either C or N termini of the basic region of the v-Jun leucine zipper gene-regulatory protein, we constructed three dimers - pCC, pCN, and pNN. All three bind specifically to the appropriately rearranged DNA recognition sites for v-Jun: ATGAcgTCAT, ATGAcgATGA, and TCATcgTCAT, respectively (Kd, ≈4 nM at 4°C). Results of DNase I footprinting provide strong support for bent recognition helices in leucine zipper protein-DNA complexes. Comparison of the results for pCC and pNN with those for pCN shows the design superiority of palindromic sequences for protein recognition.

U2 - 10.1073/pnas.90.11.4892

DO - 10.1073/pnas.90.11.4892

M3 - Article

C2 - 8506333

AN - SCOPUS:0027180990

SN - 0027-8424

VL - 90

SP - 4892

EP - 4896

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 11

ER -

Design superiority of palindromic DNA sites for site-specific recognition of proteins: Tests using protein stitchery

Abstract

Access to Document

Fingerprint

Cite this