TY - JOUR
T1 - Design superiority of palindromic DNA sites for site-specific recognition of proteins
T2 - Tests using protein stitchery
AU - Park, Changmoon
AU - Campbell, Judy L.
AU - Goddard, William A.
PY - 1993/6/1
Y1 - 1993/6/1
N2 - Using protein stitchery with appropriate attachment of cysteines linking to either C or N termini of the basic region of the v-Jun leucine zipper gene-regulatory protein, we constructed three dimers - pCC, pCN, and pNN. All three bind specifically to the appropriately rearranged DNA recognition sites for v-Jun: ATGAcgTCAT, ATGAcgATGA, and TCATcgTCAT, respectively (Kd, ≈4 nM at 4°C). Results of DNase I footprinting provide strong support for bent recognition helices in leucine zipper protein-DNA complexes. Comparison of the results for pCC and pNN with those for pCN shows the design superiority of palindromic sequences for protein recognition.
AB - Using protein stitchery with appropriate attachment of cysteines linking to either C or N termini of the basic region of the v-Jun leucine zipper gene-regulatory protein, we constructed three dimers - pCC, pCN, and pNN. All three bind specifically to the appropriately rearranged DNA recognition sites for v-Jun: ATGAcgTCAT, ATGAcgATGA, and TCATcgTCAT, respectively (Kd, ≈4 nM at 4°C). Results of DNase I footprinting provide strong support for bent recognition helices in leucine zipper protein-DNA complexes. Comparison of the results for pCC and pNN with those for pCN shows the design superiority of palindromic sequences for protein recognition.
U2 - 10.1073/pnas.90.11.4892
DO - 10.1073/pnas.90.11.4892
M3 - Article
C2 - 8506333
AN - SCOPUS:0027180990
SN - 0027-8424
VL - 90
SP - 4892
EP - 4896
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -