Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

Marlène Vayssières; Gabrielle Bourgeois; Florian Chardon; Anne Sophie Tillault; Magali Blaud

doi:10.1007/978-1-0716-1386-3_7

Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

Marlène Vayssières
, Gabrielle Bourgeois
, Florian Chardon
, Anne Sophie Tillault
, Magali Blaud

Laboratoire de Probabilités et Modèles Aléatoires
ZMBE Zentrum für Molekularbuiologie der Entzündung
Alberta RNA Research and Training Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol does not require any specific material, and the procedure is fast and sensitive.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	65-72
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-0716-1386-3_7
Publication status	Published - 1 Jan 2021

Publication series

Name	Methods in Molecular Biology
Volume	2300
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Bridge oligonucleotide
Ligation
Small RNA

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10.1007/978-1-0716-1386-3_7

Cite this

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abstract = "Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol does not require any specific material, and the procedure is fast and sensitive.",

keywords = "Bridge oligonucleotide, Ligation, Small RNA",

author = "Marl{\`e}ne Vayssi{\`e}res and Gabrielle Bourgeois and Florian Chardon and Tillault, \{Anne Sophie\} and Magali Blaud",

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T1 - Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

AU - Vayssières, Marlène

AU - Bourgeois, Gabrielle

AU - Chardon, Florian

AU - Tillault, Anne Sophie

AU - Blaud, Magali

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol does not require any specific material, and the procedure is fast and sensitive.

AB - Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol does not require any specific material, and the procedure is fast and sensitive.

KW - Bridge oligonucleotide

KW - Ligation

KW - Small RNA

U2 - 10.1007/978-1-0716-1386-3_7

DO - 10.1007/978-1-0716-1386-3_7

M3 - Chapter

C2 - 33792872

AN - SCOPUS:85103745868

T3 - Methods in Molecular Biology

SP - 65

EP - 72

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation

Abstract

Publication series

Keywords

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