TY - JOUR
T1 - Detection and labeling of small non-coding RNAs by splinted ligation
AU - Bourgeois, Gabrielle
AU - Chardon, Florian
AU - Tillault, Anne Sophie
AU - Blaud, Magali
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol doesn’t require any specific material, and the procedure is fast and sensitive.
AB - Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol doesn’t require any specific material, and the procedure is fast and sensitive.
KW - Bridge oligonucleotide
KW - Ligation
KW - Small RNA
U2 - 10.1007/978-1-4939-2547-6_7
DO - 10.1007/978-1-4939-2547-6_7
M3 - Article
C2 - 25791591
AN - SCOPUS:84925293944
SN - 1940-6029
VL - 1296
SP - 65
EP - 72
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -