TY - GEN
T1 - Dual-color 1.25-MHz Sub-70-fs source at 1.3 and 1.7 μm for multimodal 3-photon microscopy in water-transparency bands
AU - Druon, Frederic
AU - Guesmi, Khmaies
AU - Abdeladim, Lamiae
AU - Berberian, Tiphaine
AU - Rigaud, Philippe
AU - Ortas, Julia Ferrer
AU - Hanna, Marc
AU - Mahou, Pierre
AU - Livet, Jean
AU - Supatto, Willy
AU - Georges, Patrick
AU - Beaurepaire, Emmanuel
N1 - Publisher Copyright:
© 2019 IEEE.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Since its first demonstrations, two-photon microscopy has enabled discoveries in many fields of biology since it is a uniquely suited imaging method for live / deep fluorescence imaging of biological tissues with sub-cellular resolution. However, imaging depth remains a crucial limitation for investigating scattering tissues such as the brain. To overcome this limitation and access deeper areas, one recent and very promising approach consists in using three-photon excitation (3PE) and shifting the excitation to the SWIR (Short-Wavelength InfraRed) range. Indeed, scattering decreases with increasing wavelength, following a typical 1/λ3 law. However water absorption should be avoided to prevent tissue from heating, so that it was realized recently that two optimal spectral excitation windows for deep multiphoton imaging lie in the 1300 and 1700 nm regions as shown in fig. 1 [1]. We are presenting here the first dual-color SWIR source optimized for 3PE microscopy. The source is based on an OPCPA (optical parametric chirped pulse amplification) injected by a high-power Yb-fiber system. It operates at 1.25 MHz and emits concomitantly the two optimal 1.3 and 1.7 μm wavelengths with 69 fs, 65 fs pulse durations and with energies of 710 nJ and 3100 nJ respectively. These characteristics enabled us to record dual-color 3PE microscopy images in chick spinal cord, and in zebrafish and mouse brain.
AB - Since its first demonstrations, two-photon microscopy has enabled discoveries in many fields of biology since it is a uniquely suited imaging method for live / deep fluorescence imaging of biological tissues with sub-cellular resolution. However, imaging depth remains a crucial limitation for investigating scattering tissues such as the brain. To overcome this limitation and access deeper areas, one recent and very promising approach consists in using three-photon excitation (3PE) and shifting the excitation to the SWIR (Short-Wavelength InfraRed) range. Indeed, scattering decreases with increasing wavelength, following a typical 1/λ3 law. However water absorption should be avoided to prevent tissue from heating, so that it was realized recently that two optimal spectral excitation windows for deep multiphoton imaging lie in the 1300 and 1700 nm regions as shown in fig. 1 [1]. We are presenting here the first dual-color SWIR source optimized for 3PE microscopy. The source is based on an OPCPA (optical parametric chirped pulse amplification) injected by a high-power Yb-fiber system. It operates at 1.25 MHz and emits concomitantly the two optimal 1.3 and 1.7 μm wavelengths with 69 fs, 65 fs pulse durations and with energies of 710 nJ and 3100 nJ respectively. These characteristics enabled us to record dual-color 3PE microscopy images in chick spinal cord, and in zebrafish and mouse brain.
U2 - 10.1109/CLEOE-EQEC.2019.8871942
DO - 10.1109/CLEOE-EQEC.2019.8871942
M3 - Conference contribution
AN - SCOPUS:85074645273
T3 - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
BT - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019
Y2 - 23 June 2019 through 27 June 2019
ER -