Dualex: A new instrument for field measurements of epidermal ultraviolet absorbance by chlorophyll fluorescence

Yves Goulas, Zoran G. Cerovic, Aurélie Cartelat, Ismaël Moya

Research output: Contribution to journalArticlepeer-review

Abstract

Dualex (dual excitation) is a field-portable instrument, hereby described, for the assessment of polyphenolic compounds in leaves from the measurement of UV absorbance of the leaf epidermis by double excitation of chlorophyll fluorescence. The instrument takes advantage of a feedback loop that equalizes the fluorescence level induced by a reference red light to the UV-light-induced fluorescence level. This allows quick measurement from attached leaves even under field conditions. The use of light-emitting diodes and of a leaf-clip configuration makes Dualex a user-friendly instrument with potential applications in ecophysiological research, light climate analysis, agriculture, forestry, horticulture, pest management, selection of medicinal plants, and wherever accumulation of leaf polyphenolics is involved in plant responses to the environment.

Original languageEnglish
Pages (from-to)4488-4496
Number of pages9
JournalApplied Optics
Volume43
Issue number23
DOIs
Publication statusPublished - 10 Aug 2004
Externally publishedYes

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