Effects of Sequence and Base Composition on the CD and TDS Profiles of i-DNA

Nunzia Iaccarino; Mingpan Cheng; Dehui Qiu; Bruno Pagano; Jussara Amato; Anna Di Porzio; Jun Zhou; Antonio Randazzo; Jean Louis Mergny

doi:10.1002/anie.202016822

Effects of Sequence and Base Composition on the CD and TDS Profiles of i-DNA

Nunzia Iaccarino, Mingpan Cheng, Dehui Qiu, Bruno Pagano, Jussara Amato, Anna Di Porzio, Jun Zhou, Antonio Randazzo, Jean Louis Mergny

Research output: Contribution to journal › Article › peer-review

Abstract

The i-motif DNA, also known as i-DNA, is a non-canonical DNA secondary structure formed by cytosine-rich sequences, consisting of two intercalated parallel-stranded duplexes held together by hemi-protonated cytosine–cytosine⁺ (C:C⁺) base pairs. The growing interest in the i-DNA structure as a target in anticancer therapy increases the need for tools for a rapid and meaningful interpretation of the spectroscopic data of i-DNA samples. Herein, we analyzed the circular dichroism (CD) and thermal difference UV-absorbance spectra (TDS) of 255 DNA sequences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i-DNA-forming sequences.

Original language	English
Pages (from-to)	10295-10303
Number of pages	9
Journal	Angewandte Chemie - International Edition
Volume	60
Issue number	18
DOIs	https://doi.org/10.1002/anie.202016822
Publication status	Published - 26 Apr 2021

Keywords

DNA
circular dichroism
i-motif
multivariate data analysis
thermal difference spectroscopy

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10.1002/anie.202016822

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title = "Effects of Sequence and Base Composition on the CD and TDS Profiles of i-DNA",

abstract = "The i-motif DNA, also known as i-DNA, is a non-canonical DNA secondary structure formed by cytosine-rich sequences, consisting of two intercalated parallel-stranded duplexes held together by hemi-protonated cytosine–cytosine+ (C:C+) base pairs. The growing interest in the i-DNA structure as a target in anticancer therapy increases the need for tools for a rapid and meaningful interpretation of the spectroscopic data of i-DNA samples. Herein, we analyzed the circular dichroism (CD) and thermal difference UV-absorbance spectra (TDS) of 255 DNA sequences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i-DNA-forming sequences.",

keywords = "DNA, circular dichroism, i-motif, multivariate data analysis, thermal difference spectroscopy",

author = "Nunzia Iaccarino and Mingpan Cheng and Dehui Qiu and Bruno Pagano and Jussara Amato and \{Di Porzio\}, Anna and Jun Zhou and Antonio Randazzo and Mergny, \{Jean Louis\}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH",

year = "2021",

month = apr,

day = "26",

doi = "10.1002/anie.202016822",

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T1 - Effects of Sequence and Base Composition on the CD and TDS Profiles of i-DNA

AU - Iaccarino, Nunzia

AU - Cheng, Mingpan

AU - Qiu, Dehui

AU - Pagano, Bruno

AU - Amato, Jussara

AU - Di Porzio, Anna

AU - Zhou, Jun

AU - Randazzo, Antonio

AU - Mergny, Jean Louis

PY - 2021/4/26

Y1 - 2021/4/26

N2 - The i-motif DNA, also known as i-DNA, is a non-canonical DNA secondary structure formed by cytosine-rich sequences, consisting of two intercalated parallel-stranded duplexes held together by hemi-protonated cytosine–cytosine+ (C:C+) base pairs. The growing interest in the i-DNA structure as a target in anticancer therapy increases the need for tools for a rapid and meaningful interpretation of the spectroscopic data of i-DNA samples. Herein, we analyzed the circular dichroism (CD) and thermal difference UV-absorbance spectra (TDS) of 255 DNA sequences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i-DNA-forming sequences.

AB - The i-motif DNA, also known as i-DNA, is a non-canonical DNA secondary structure formed by cytosine-rich sequences, consisting of two intercalated parallel-stranded duplexes held together by hemi-protonated cytosine–cytosine+ (C:C+) base pairs. The growing interest in the i-DNA structure as a target in anticancer therapy increases the need for tools for a rapid and meaningful interpretation of the spectroscopic data of i-DNA samples. Herein, we analyzed the circular dichroism (CD) and thermal difference UV-absorbance spectra (TDS) of 255 DNA sequences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i-DNA-forming sequences.

KW - DNA

KW - circular dichroism

KW - i-motif

KW - multivariate data analysis

KW - thermal difference spectroscopy

U2 - 10.1002/anie.202016822

DO - 10.1002/anie.202016822

M3 - Article

C2 - 33617090

AN - SCOPUS:85102928086

SN - 1433-7851

VL - 60

SP - 10295

EP - 10303

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 18

ER -

Effects of Sequence and Base Composition on the CD and TDS Profiles of i-DNA

Abstract

Keywords

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