Elastic and electrostatic model for DNA in rotation-extension experiments

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Abstract

We present a self-contained theory for the mechanical response of DNA in extension-rotation single molecule experiments. The theory is based on the elasticity of the double-helix and the electrostatic repulsion between two DNA duplex. The configuration of the molecule at large imposed rotation is assumed to comprise two phases, linear and superhelical DNA. Thermal fluctuations are accounted for in the linear phase and electrostatic repulsion is treated in the superhelical phase. This analytical model enables the computation of the supercoiling radius and angle of DNA during experiments. The torsional stress in the molecule and the slope of the linear region of the experimental curve are also predicted and compared successfully with experimental data.

Original languageEnglish
Title of host publicationIUTAM Symposium on Cellular, Molecular and Tissue Mechanics - Proceedings of the IUTAM Symposium
Pages113-122
Number of pages10
DOIs
Publication statusPublished - 1 Dec 2010
EventIUTAM Symposium on Cellular, Molecular and Tissue Mechanics - Woods Hole, MA, United States
Duration: 18 Jun 200821 Jun 2008

Publication series

NameIUTAM Bookseries
Volume16
ISSN (Print)1875-3507

Conference

ConferenceIUTAM Symposium on Cellular, Molecular and Tissue Mechanics
Country/TerritoryUnited States
CityWoods Hole, MA
Period18/06/0821/06/08

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