TY - JOUR
T1 - Essential Roles of Dopamine and Serotonin in Tooth Repair
T2 - Functional Interplay between Odontogenic Stem Cells and Platelets
AU - Baudry, Anne
AU - Alleaume-Butaux, Aurélie
AU - Dimitrova-Nakov, Sasha
AU - Goldberg, Michel
AU - Schneider, Benoît
AU - Launay, Jean Marie
AU - Kellermann, Odile
N1 - Publisher Copyright:
© 2015 AlphaMed Press.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Characterizing stem cell intrinsic functions is an ongoing challenge for cell therapies. Here, we report that two independent A4 and H8 stem cell lines isolated from mouse molar pulp display the overall functions of bioaminergic cells. Both clones produce neurotrophins and synthesize, catabolize, store, and transport serotonin (5-hydroxytryptamine [5-HT]) and dopamine (DA). They express 5-HT1D,2B,7 and D1,3 autoreceptors, which render pulpal stem cells competent to respond to circulating 5-HT and DA. We show that injury-activated platelets are the source of systemic 5-HT and DA necessary for dental repair since natural dentin reparation is impaired in two rat models with monoamine storage-deficient blood platelets. Moreover, selective inhibition of either D1, D3, 5-HT2B, or 5-HT7 receptor within the pulp of wild-type rat molars after lesion alters the reparative process. Altogether our data argue that 5-HT and DA coreleased by pulp injury-activated platelets are critical for stem cell-mediated dental repair through 5-HT and DA receptor signalings.
AB - Characterizing stem cell intrinsic functions is an ongoing challenge for cell therapies. Here, we report that two independent A4 and H8 stem cell lines isolated from mouse molar pulp display the overall functions of bioaminergic cells. Both clones produce neurotrophins and synthesize, catabolize, store, and transport serotonin (5-hydroxytryptamine [5-HT]) and dopamine (DA). They express 5-HT1D,2B,7 and D1,3 autoreceptors, which render pulpal stem cells competent to respond to circulating 5-HT and DA. We show that injury-activated platelets are the source of systemic 5-HT and DA necessary for dental repair since natural dentin reparation is impaired in two rat models with monoamine storage-deficient blood platelets. Moreover, selective inhibition of either D1, D3, 5-HT2B, or 5-HT7 receptor within the pulp of wild-type rat molars after lesion alters the reparative process. Altogether our data argue that 5-HT and DA coreleased by pulp injury-activated platelets are critical for stem cell-mediated dental repair through 5-HT and DA receptor signalings.
KW - Dental pulp stem cells
KW - Dental repair
KW - Dopamine
KW - Serotonin
KW - Signaling
U2 - 10.1002/stem.2037
DO - 10.1002/stem.2037
M3 - Article
C2 - 25865138
AN - SCOPUS:84937628343
SN - 1066-5099
VL - 33
SP - 2586
EP - 2595
JO - Stem Cells
JF - Stem Cells
IS - 8
ER -