Evaluation of Slowfade Diamond as a buffer for STORM microscopy

Hadjer Boukhatem; Beatrice Durel; Manon Raimbault; Audrey Laurent; Nicolas Olivier

doi:10.1364/BOE.473463

Evaluation of Slowfade Diamond as a buffer for STORM microscopy

Hadjer Boukhatem
, Beatrice Durel
, Manon Raimbault
, Audrey Laurent
, Nicolas Olivier

Institut Polytechnique de Paris
INSERM U869
Laboratoire de Probabilités et Modèles Aléatoires

Research output: Contribution to journal › Article › peer-review

Abstract

We study the potential of the commercial mounting medium Slowfade diamond as a buffer for STORM microscopy. We show that although it does not work with the popular far-red dyes typically used for STORM imaging, such as Alexa Fluor 647, it performs really well with a wide variety of green-excited dyes such as Alexa Fluor 532, Alexa Fluor 555 or CF 568. Moreover, imaging can be performed several months after the samples are mounted in this environment and kept in the fridge, providing a convenient way to preserve samples for STORM imaging, as well as to keep calibration samples, for example for metrology or teaching in particular in imaging facilities.

Original language	English
Pages (from-to)	550-558
Number of pages	9
Journal	Biomedical Optics Express
Volume	14
Issue number	2
DOIs	https://doi.org/10.1364/BOE.473463
Publication status	Published - 1 Feb 2023

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title = "Evaluation of Slowfade Diamond as a buffer for STORM microscopy",

abstract = "We study the potential of the commercial mounting medium Slowfade diamond as a buffer for STORM microscopy. We show that although it does not work with the popular far-red dyes typically used for STORM imaging, such as Alexa Fluor 647, it performs really well with a wide variety of green-excited dyes such as Alexa Fluor 532, Alexa Fluor 555 or CF 568. Moreover, imaging can be performed several months after the samples are mounted in this environment and kept in the fridge, providing a convenient way to preserve samples for STORM imaging, as well as to keep calibration samples, for example for metrology or teaching in particular in imaging facilities.",

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AU - Olivier, Nicolas

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AB - We study the potential of the commercial mounting medium Slowfade diamond as a buffer for STORM microscopy. We show that although it does not work with the popular far-red dyes typically used for STORM imaging, such as Alexa Fluor 647, it performs really well with a wide variety of green-excited dyes such as Alexa Fluor 532, Alexa Fluor 555 or CF 568. Moreover, imaging can be performed several months after the samples are mounted in this environment and kept in the fridge, providing a convenient way to preserve samples for STORM imaging, as well as to keep calibration samples, for example for metrology or teaching in particular in imaging facilities.

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Evaluation of Slowfade Diamond as a buffer for STORM microscopy

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