Evidence for a unique Rieske iron-sulphur centre in Heliobacterium chlorum

Ursula Liebl; A. William Rutherford; Wolfgang Nitschke

doi:10.1016/0014-5793(90)80608-L

Evidence for a unique Rieske iron-sulphur centre in Heliobacterium chlorum

Ursula Liebl
, A. William Rutherford
, Wolfgang Nitschke

Institut Pierre Simon Laplace, CNRS and CEA

Research output: Contribution to journal › Article › peer-review

Abstract

An iron sulphur centre (g_z = 2.035, g_y =1.89, g_x =1.81), which can be observed in both whole cells and isolated plasma membranes of Heliobacterium chlorum, was identified as a Rieske centre on the basis of its sensitivity to the inhibitors 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone (DBMIB), 5-(n-undecyl)-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) and stigmatellin as well as on the basis of its orientation (gx, is oriented perpendicular and g_y is oriented parallel to the membrane plane). Its midpoint potential is unusually low (E_m,7= +120 ± 10 mV) and does not depend on pH in the range between pH 6.5 and pH 8.0. The effects of the inhibitors on the EPR spectrum are altered compared to Rieske centres in other systems. The significance of the low E_m is discussed with regard to the overall midpoint potentials of the electron transfer chain in Heliobacterium chlorum.

Original language	English
Pages (from-to)	427-430
Number of pages	4
Journal	FEBS Letters
Volume	261
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(90)80608-L
Publication status	Published - 26 Feb 1990
Externally published	Yes

Keywords

Cytochrome bc-complex
Electron paramagnetic resonance spectrometry
Heliobacterium chlorum
Inhibitor
Q-site
Rieske center

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10.1016/0014-5793(90)80608-L

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title = "Evidence for a unique Rieske iron-sulphur centre in Heliobacterium chlorum",

abstract = "An iron sulphur centre (gz = 2.035, gy =1.89, gx =1.81), which can be observed in both whole cells and isolated plasma membranes of Heliobacterium chlorum, was identified as a Rieske centre on the basis of its sensitivity to the inhibitors 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone (DBMIB), 5-(n-undecyl)-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) and stigmatellin as well as on the basis of its orientation (gx, is oriented perpendicular and gy is oriented parallel to the membrane plane). Its midpoint potential is unusually low (Em,7= +120 ± 10 mV) and does not depend on pH in the range between pH 6.5 and pH 8.0. The effects of the inhibitors on the EPR spectrum are altered compared to Rieske centres in other systems. The significance of the low Em is discussed with regard to the overall midpoint potentials of the electron transfer chain in Heliobacterium chlorum.",

keywords = "Cytochrome bc-complex, Electron paramagnetic resonance spectrometry, Heliobacterium chlorum, Inhibitor, Q-site, Rieske center",

author = "Ursula Liebl and Rutherford, \{A. William\} and Wolfgang Nitschke",

year = "1990",

month = feb,

day = "26",

doi = "10.1016/0014-5793(90)80608-L",

language = "English",

volume = "261",

pages = "427--430",

journal = "FEBS Letters",

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T1 - Evidence for a unique Rieske iron-sulphur centre in Heliobacterium chlorum

AU - Liebl, Ursula

AU - Rutherford, A. William

AU - Nitschke, Wolfgang

PY - 1990/2/26

Y1 - 1990/2/26

N2 - An iron sulphur centre (gz = 2.035, gy =1.89, gx =1.81), which can be observed in both whole cells and isolated plasma membranes of Heliobacterium chlorum, was identified as a Rieske centre on the basis of its sensitivity to the inhibitors 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone (DBMIB), 5-(n-undecyl)-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) and stigmatellin as well as on the basis of its orientation (gx, is oriented perpendicular and gy is oriented parallel to the membrane plane). Its midpoint potential is unusually low (Em,7= +120 ± 10 mV) and does not depend on pH in the range between pH 6.5 and pH 8.0. The effects of the inhibitors on the EPR spectrum are altered compared to Rieske centres in other systems. The significance of the low Em is discussed with regard to the overall midpoint potentials of the electron transfer chain in Heliobacterium chlorum.

AB - An iron sulphur centre (gz = 2.035, gy =1.89, gx =1.81), which can be observed in both whole cells and isolated plasma membranes of Heliobacterium chlorum, was identified as a Rieske centre on the basis of its sensitivity to the inhibitors 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone (DBMIB), 5-(n-undecyl)-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) and stigmatellin as well as on the basis of its orientation (gx, is oriented perpendicular and gy is oriented parallel to the membrane plane). Its midpoint potential is unusually low (Em,7= +120 ± 10 mV) and does not depend on pH in the range between pH 6.5 and pH 8.0. The effects of the inhibitors on the EPR spectrum are altered compared to Rieske centres in other systems. The significance of the low Em is discussed with regard to the overall midpoint potentials of the electron transfer chain in Heliobacterium chlorum.

KW - Cytochrome bc-complex

KW - Electron paramagnetic resonance spectrometry

KW - Heliobacterium chlorum

KW - Inhibitor

KW - Q-site

KW - Rieske center

UR - https://www.scopus.com/pages/publications/0025019450

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DO - 10.1016/0014-5793(90)80608-L

M3 - Article

AN - SCOPUS:0025019450

SN - 0014-5793

VL - 261

SP - 427

EP - 430

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Evidence for a unique Rieske iron-sulphur centre in Heliobacterium chlorum

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Keywords

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