Exploration of single molecule events in a haloperoxidase and its biomimic: Localization of halogenation activity

Virginia Martínez Martínez; Gert De Cremer; Maarten B.J. Roeffaers; Michel Sliwa; Mukulesh Baruah; Dirk E. De Vos; Johan Hofkens; Bert F. Sels

doi:10.1021/ja804606m

Exploration of single molecule events in a haloperoxidase and its biomimic: Localization of halogenation activity

Virginia Martínez Martínez, Gert De Cremer, Maarten B.J. Roeffaers, Michel Sliwa, Mukulesh Baruah, Dirk E. De Vos, Johan Hofkens, Bert F. Sels

KU Leuven

Research output: Contribution to journal › Article › peer-review

Abstract

In situ observation of single oxidation/halogenation events by catalytically generated hypobromite species using single molecule fluorescence microscopy allows monitoring of the diffusion behavior of these halonium species from the catalyst into the bulk solution. The fluororgenic probe specifically reacts with hypohalites, yielding fluorescein that can be detected with single molecule sensitivity. It was found for two investigated catalysts (Curvularia verruculosa enzymes and tungstate-exchanged LDH crystals) that in steady-state conditions hypobromite is able to diffuse over 800 nm in the bulk solution before it oxidizes organic substrates.

Original language	English
Pages (from-to)	13192-13193
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	130
Issue number	40
DOIs	https://doi.org/10.1021/ja804606m
Publication status	Published - 8 Oct 2008
Externally published	Yes

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@article{c14c8cfbc2374a1a9f9bb1ac15383e10,

title = "Exploration of single molecule events in a haloperoxidase and its biomimic: Localization of halogenation activity",

abstract = "In situ observation of single oxidation/halogenation events by catalytically generated hypobromite species using single molecule fluorescence microscopy allows monitoring of the diffusion behavior of these halonium species from the catalyst into the bulk solution. The fluororgenic probe specifically reacts with hypohalites, yielding fluorescein that can be detected with single molecule sensitivity. It was found for two investigated catalysts (Curvularia verruculosa enzymes and tungstate-exchanged LDH crystals) that in steady-state conditions hypobromite is able to diffuse over 800 nm in the bulk solution before it oxidizes organic substrates.",

author = "Mart{\'i}nez, \{Virginia Mart{\'i}nez\} and \{De Cremer\}, Gert and Roeffaers, \{Maarten B.J.\} and Michel Sliwa and Mukulesh Baruah and \{De Vos\}, \{Dirk E.\} and Johan Hofkens and Sels, \{Bert F.\}",

year = "2008",

month = oct,

day = "8",

doi = "10.1021/ja804606m",

language = "English",

volume = "130",

pages = "13192--13193",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

number = "40",

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TY - JOUR

T1 - Exploration of single molecule events in a haloperoxidase and its biomimic

T2 - Localization of halogenation activity

AU - Martínez, Virginia Martínez

AU - De Cremer, Gert

AU - Roeffaers, Maarten B.J.

AU - Sliwa, Michel

AU - Baruah, Mukulesh

AU - De Vos, Dirk E.

AU - Hofkens, Johan

AU - Sels, Bert F.

PY - 2008/10/8

Y1 - 2008/10/8

N2 - In situ observation of single oxidation/halogenation events by catalytically generated hypobromite species using single molecule fluorescence microscopy allows monitoring of the diffusion behavior of these halonium species from the catalyst into the bulk solution. The fluororgenic probe specifically reacts with hypohalites, yielding fluorescein that can be detected with single molecule sensitivity. It was found for two investigated catalysts (Curvularia verruculosa enzymes and tungstate-exchanged LDH crystals) that in steady-state conditions hypobromite is able to diffuse over 800 nm in the bulk solution before it oxidizes organic substrates.

AB - In situ observation of single oxidation/halogenation events by catalytically generated hypobromite species using single molecule fluorescence microscopy allows monitoring of the diffusion behavior of these halonium species from the catalyst into the bulk solution. The fluororgenic probe specifically reacts with hypohalites, yielding fluorescein that can be detected with single molecule sensitivity. It was found for two investigated catalysts (Curvularia verruculosa enzymes and tungstate-exchanged LDH crystals) that in steady-state conditions hypobromite is able to diffuse over 800 nm in the bulk solution before it oxidizes organic substrates.

U2 - 10.1021/ja804606m

DO - 10.1021/ja804606m

M3 - Article

C2 - 18785736

AN - SCOPUS:53549086184

SN - 0002-7863

VL - 130

SP - 13192

EP - 13193

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 40

ER -

Exploration of single molecule events in a haloperoxidase and its biomimic: Localization of halogenation activity

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