Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

Vincent Maioli; Antoine Boniface; Pierre Mahou; Júlia Ferrer Ortas; Lamiae Abdeladim; Emmanuel Beaurepaire; Willy Supatto

Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

Vincent Maioli
, Antoine Boniface
, Pierre Mahou
, Júlia Ferrer Ortas
, Lamiae Abdeladim
, Emmanuel Beaurepaire
, Willy Supatto

Institut Polytechnique de Paris

Research output: Contribution to journal › Conference article › peer-review

Abstract

We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.

Original language	English
Article number	EM2B.2
Journal	Optics InfoBase Conference Papers
Publication status	Published - 1 Jan 2021
Event	2021 European Conferences on Biomedical Optics, ECBO 2021 - Virtual, Online, Germany Duration: 20 Jun 2021 → 24 Jun 2021

Cite this

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title = "Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate",

abstract = "We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.",

author = "Vincent Maioli and Antoine Boniface and Pierre Mahou and Ortas, \{J{\'u}lia Ferrer\} and Lamiae Abdeladim and Emmanuel Beaurepaire and Willy Supatto",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s); 2021 European Conferences on Biomedical Optics, ECBO 2021 ; Conference date: 20-06-2021 Through 24-06-2021",

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TY - JOUR

T1 - Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

AU - Maioli, Vincent

AU - Boniface, Antoine

AU - Mahou, Pierre

AU - Ortas, Júlia Ferrer

AU - Abdeladim, Lamiae

AU - Beaurepaire, Emmanuel

AU - Supatto, Willy

PY - 2021/1/1

Y1 - 2021/1/1

N2 - We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.

AB - We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.

M3 - Conference article

AN - SCOPUS:85119439652

SN - 2162-2701

JO - Optics InfoBase Conference Papers

JF - Optics InfoBase Conference Papers

M1 - EM2B.2

T2 - 2021 European Conferences on Biomedical Optics, ECBO 2021

Y2 - 20 June 2021 through 24 June 2021

ER -

Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

Abstract

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