Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency

Vincent Maioli; Antoine Boniface; Pierre Mahou; Júlia Ferrer Ortas; Lamiae Abdeladim; Emmanuel Beaurepaire; Willy Supatto

doi:10.1364/BOE.400113

Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto

Institut Polytechnique de Paris

Research output: Contribution to journal › Article › peer-review

Abstract

Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast imaging in vivo. However, photoperturbation in multiphoton light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe of linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency and wavelength, we derive guidelines to find the best balance between signal and photoperturbation. We then demonstrate one order-of-magnitude signal enhancement over previous implementations by optimizing the laser pulse frequency. These results open new opportunities for fast live tissue imaging.

Original language	English
Pages (from-to)	6012-6026
Number of pages	15
Journal	Biomedical Optics Express
Volume	11
Issue number	10
DOIs	https://doi.org/10.1364/BOE.400113
Publication status	Published - 1 Oct 2020

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title = "Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency",

abstract = "Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast imaging in vivo. However, photoperturbation in multiphoton light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe of linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency and wavelength, we derive guidelines to find the best balance between signal and photoperturbation. We then demonstrate one order-of-magnitude signal enhancement over previous implementations by optimizing the laser pulse frequency. These results open new opportunities for fast live tissue imaging.",

author = "Vincent Maioli and Antoine Boniface and Pierre Mahou and Ortas, \{J{\'u}lia Ferrer\} and Lamiae Abdeladim and Emmanuel Beaurepaire and Willy Supatto",

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AU - Maioli, Vincent

AU - Boniface, Antoine

AU - Mahou, Pierre

AU - Ortas, Júlia Ferrer

AU - Abdeladim, Lamiae

AU - Beaurepaire, Emmanuel

AU - Supatto, Willy

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast imaging in vivo. However, photoperturbation in multiphoton light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe of linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency and wavelength, we derive guidelines to find the best balance between signal and photoperturbation. We then demonstrate one order-of-magnitude signal enhancement over previous implementations by optimizing the laser pulse frequency. These results open new opportunities for fast live tissue imaging.

AB - Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast imaging in vivo. However, photoperturbation in multiphoton light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe of linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency and wavelength, we derive guidelines to find the best balance between signal and photoperturbation. We then demonstrate one order-of-magnitude signal enhancement over previous implementations by optimizing the laser pulse frequency. These results open new opportunities for fast live tissue imaging.

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DO - 10.1364/BOE.400113

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EP - 6026

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 10

ER -

Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency

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