Abstract
We characterized the effect of femtosecond pulse trains on gastrulating Drosophila embryos using two-photon-excited fluorescence (2PEF) and third-harmonic generation (THG) microscopy. Femtosecond pulses can be used to perform controlled intravital microdissections that alter the embryo structural integrity but do not significantly perturb cytoskeleton dynamics in adjacent cells. Such targeted ablations can be used to remotely perturb cell movements in developing embryos. The transparent combination of femtosecond pulse-induced ablation with nonlinear microscopy can be used to analyze in vivo the effect of cell and tissue deformations.
| Original language | English |
|---|---|
| Pages (from-to) | 207-216 |
| Number of pages | 10 |
| Journal | Medical Laser Application |
| Volume | 20 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 14 Oct 2005 |
Keywords
- Drosophila embryo development
- Femtosecond pulse-induced ablation
- Third-harmonic generation microscopy
- Two-photon-excited fluorescence microscopy