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Fluorescence resonance energy transfer using lanthanide-ion doped oxide nanoparticles as donors

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Abstract

We have demonstrated fluorescence resonance energy transfer (FRET) between lanthanide-ion doped oxide nanoparticles acting as donors and organic acceptor molecules (Cy5). Due to the long nanoparticle lifetime and the large Stokes shift between nanoparticle absorption and emission, unambiguous and precise FRET measurements can be performed despite the presence of large free acceptor concentrations. We determined FRET efficiencies as a function of Cy5 concentration which are in very good agreement with a multiple acceptor - multiple donor calculation.

Original languageEnglish
Title of host publicationColloidal Quantum Dots for Biomedical Applications
DOIs
Publication statusPublished - 26 Jun 2006
EventColloidal Quantum Dots for Biomedical Applications - San Jose, CA, United States
Duration: 22 Jan 200624 Jan 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6096
ISSN (Print)1605-7422

Conference

ConferenceColloidal Quantum Dots for Biomedical Applications
Country/TerritoryUnited States
CitySan Jose, CA
Period22/01/0624/01/06

Keywords

  • Lanthanide
  • Nanoparticles
  • Resonance energy transfer

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