Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Second harmonic generation (SHG) is a powerful technique to observe fibrillar collagen without any staining and with a good contrast. More information about the molecular structure of collagen fibrils in tissues and their 3D distribution can be gained with polarization-resolved SHG imaging. Nevertheless, strong focusing is required for effective imaging and light propagation in tissues is complex and not thoroughly understood yet, preventing accurate and reproducible measurements. Theoretical analysis, vectorial numerical simulations and experiments were implemented to understand how the SHG signal builds up and how geometrical parameters affect polarization-resolved measurements in homogeneous collagen-rich tissues.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XVI
EditorsPeter T. C. So, Karsten Konig, Ammasi Periasamy, Karsten Konig
PublisherSPIE
ISBN (Electronic)9781628419467
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences XVI - San Francisco, United States
Duration: 14 Feb 201616 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9712
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XVI
Country/TerritoryUnited States
CitySan Francisco
Period14/02/1616/02/16

Keywords

  • Multiphoton microscopy
  • SHG imaging
  • collagen
  • cornea
  • polarimetry
  • tendon
  • vectorial numerical calculations

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