Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence

C. Rougeulle; L. Colleaux; B. Dujon; P. Avner

doi:10.1007/BF00357001

Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence

C. Rougeulle, L. Colleaux, B. Dujon, P. Avner

Institut Pasteur, Paris

Research output: Contribution to journal › Article › peer-review

Abstract

The Xist sequence has several characteristics that make it a potential candidate for the X-inactivation center. To investigate the role of Xist and adjacent sequences lying within the X-inactivation center candidate region, a 460-kb region surrounding the murine Xist sequence has been arrayed in lambda contigs with a combination of IRS-PCR-based hybridization and YAC fragmentation. The orientation of the Xist sequence in relation to the telomere and centromere of the X Chromosome (Chr) has been established with this contig and shown to be inverted compared to that in human.

Original language	English
Pages (from-to)	416-423
Number of pages	8
Journal	Mammalian Genome
Volume	5
Issue number	7
DOIs	https://doi.org/10.1007/BF00357001
Publication status	Published - 1 Jul 1994
Externally published	Yes

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title = "Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence",

abstract = "The Xist sequence has several characteristics that make it a potential candidate for the X-inactivation center. To investigate the role of Xist and adjacent sequences lying within the X-inactivation center candidate region, a 460-kb region surrounding the murine Xist sequence has been arrayed in lambda contigs with a combination of IRS-PCR-based hybridization and YAC fragmentation. The orientation of the Xist sequence in relation to the telomere and centromere of the X Chromosome (Chr) has been established with this contig and shown to be inverted compared to that in human.",

author = "C. Rougeulle and L. Colleaux and B. Dujon and P. Avner",

year = "1994",

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AU - Rougeulle, C.

AU - Colleaux, L.

AU - Dujon, B.

AU - Avner, P.

PY - 1994/7/1

Y1 - 1994/7/1

N2 - The Xist sequence has several characteristics that make it a potential candidate for the X-inactivation center. To investigate the role of Xist and adjacent sequences lying within the X-inactivation center candidate region, a 460-kb region surrounding the murine Xist sequence has been arrayed in lambda contigs with a combination of IRS-PCR-based hybridization and YAC fragmentation. The orientation of the Xist sequence in relation to the telomere and centromere of the X Chromosome (Chr) has been established with this contig and shown to be inverted compared to that in human.

AB - The Xist sequence has several characteristics that make it a potential candidate for the X-inactivation center. To investigate the role of Xist and adjacent sequences lying within the X-inactivation center candidate region, a 460-kb region surrounding the murine Xist sequence has been arrayed in lambda contigs with a combination of IRS-PCR-based hybridization and YAC fragmentation. The orientation of the Xist sequence in relation to the telomere and centromere of the X Chromosome (Chr) has been established with this contig and shown to be inverted compared to that in human.

U2 - 10.1007/BF00357001

DO - 10.1007/BF00357001

M3 - Article

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AN - SCOPUS:0028464352

SN - 0938-8990

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JO - Mammalian Genome

JF - Mammalian Genome

IS - 7

ER -

Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence

Abstract

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