High localization, focal depth and contrast by means of nonlinear Bessel Beams

We show an experimental and computational comparison between the resolution power, the contrast and the focal depth of a nonlinearly propagated diffraction-free beam and of other beams (a linear and a nonlinearly propagated Gaussian pulse): launching a nondiffractive Bessel pulse in a solution of Coumarine 120 in methanol creates a high contrast, 40 mm long, 10 μm width fluorescence channel excited by 3-photon absorption process. This fluorescence channel exhibits the same contrast and resolution of a tightly focused Gaussian pulse, but reaches a focal depth that outclasses by orders of magnitude that reached by an equivalent Gaussian pulse.