TY - JOUR
T1 - High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project
T2 - One size fits all?
AU - Leulliot, Nicolas
AU - Trésaugues, Lionel
AU - Bremang, Michael
AU - Sorel, Isabelle
AU - Ulryck, Nathalie
AU - Graille, Marc
AU - Aboulfath, Ilham
AU - Poupon, Anne
AU - Liger, Dominique
AU - Quevillon-Cheruel, Sophie
AU - Janin, Joël
AU - Van Tilbeurgh, Herman
PY - 2005/6/1
Y1 - 2005/6/1
N2 - Crystallization has long been regarded as one of the major bottlenecks in high-throughput structural determination by X-ray crystallography. Structural genomics projects have addressed this issue by using robots to set up automated crystal screens using nanodrop technology. This has moved the bottleneck from obtaining the first crystal hit to obtaining diffraction-quality crystals, as crystal optimization is a notoriously slow process that is difficult to automatize. This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples.
AB - Crystallization has long been regarded as one of the major bottlenecks in high-throughput structural determination by X-ray crystallography. Structural genomics projects have addressed this issue by using robots to set up automated crystal screens using nanodrop technology. This has moved the bottleneck from obtaining the first crystal hit to obtaining diffraction-quality crystals, as crystal optimization is a notoriously slow process that is difficult to automatize. This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples.
U2 - 10.1107/S0907444905000028
DO - 10.1107/S0907444905000028
M3 - Article
C2 - 15930617
AN - SCOPUS:23844436693
SN - 0907-4449
VL - 61
SP - 664
EP - 670
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 6
ER -