Identification of trinucleotide repeat ligands with a FRET melting assay

Samir Amrane; Anne De Cian; Frédéric Rosu; Markus Kaiser; Edwin De Pauw; Marie Paule Teulade-Fichou; Jean Louis Mergny

doi:10.1002/cbic.200800062

Identification of trinucleotide repeat ligands with a FRET melting assay

Samir Amrane
, Anne De Cian
, Frédéric Rosu
, Markus Kaiser
, Edwin De Pauw
, Marie Paule Teulade-Fichou
, Jean Louis Mergny

CNRS/Museum National d'Histoire Naturelle/IRD/UPMC
University of Liège
Centre national de la recherche scientifique

Research output: Contribution to journal › Article › peer-review

Abstract

DNA hairpin structures formed within a repeated tract might be a causative factor for triplet expansion observed in several debilitating diseases. We have designed and used a fluorescence resonance energy transfer (FRET) melting assay to screen for ligands that bind specifically to the CNG triplet repeats. Using this assay, we screened a panel of 33 chemicals that were previously designed to bind DNA or RNA secondary structures. Remarkably, we found that macrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif.

Original language	English
Pages (from-to)	1229-1234
Number of pages	6
Journal	ChemBioChem
Volume	9
Issue number	8
DOIs	https://doi.org/10.1002/cbic.200800062
Publication status	Published - 23 May 2008
Externally published	Yes

Keywords

DNA
DNA ligands
DNA recognition
FRET
Nucleic acids

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10.1002/cbic.200800062

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abstract = "DNA hairpin structures formed within a repeated tract might be a causative factor for triplet expansion observed in several debilitating diseases. We have designed and used a fluorescence resonance energy transfer (FRET) melting assay to screen for ligands that bind specifically to the CNG triplet repeats. Using this assay, we screened a panel of 33 chemicals that were previously designed to bind DNA or RNA secondary structures. Remarkably, we found that macrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif.",

keywords = "DNA, DNA ligands, DNA recognition, FRET, Nucleic acids",

author = "Samir Amrane and \{De Cian\}, Anne and Fr{\'e}d{\'e}ric Rosu and Markus Kaiser and \{De Pauw\}, Edwin and Teulade-Fichou, \{Marie Paule\} and Mergny, \{Jean Louis\}",

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AU - Amrane, Samir

AU - De Cian, Anne

AU - Rosu, Frédéric

AU - Kaiser, Markus

AU - De Pauw, Edwin

AU - Teulade-Fichou, Marie Paule

AU - Mergny, Jean Louis

PY - 2008/5/23

Y1 - 2008/5/23

N2 - DNA hairpin structures formed within a repeated tract might be a causative factor for triplet expansion observed in several debilitating diseases. We have designed and used a fluorescence resonance energy transfer (FRET) melting assay to screen for ligands that bind specifically to the CNG triplet repeats. Using this assay, we screened a panel of 33 chemicals that were previously designed to bind DNA or RNA secondary structures. Remarkably, we found that macrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif.

AB - DNA hairpin structures formed within a repeated tract might be a causative factor for triplet expansion observed in several debilitating diseases. We have designed and used a fluorescence resonance energy transfer (FRET) melting assay to screen for ligands that bind specifically to the CNG triplet repeats. Using this assay, we screened a panel of 33 chemicals that were previously designed to bind DNA or RNA secondary structures. Remarkably, we found that macrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif.

KW - DNA

KW - DNA ligands

KW - DNA recognition

KW - FRET

KW - Nucleic acids

UR - https://www.scopus.com/pages/publications/49249089906

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M3 - Article

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SN - 1439-4227

VL - 9

SP - 1229

EP - 1234

JO - ChemBioChem

JF - ChemBioChem

IS - 8

ER -

Identification of trinucleotide repeat ligands with a FRET melting assay

Abstract

Keywords

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