Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: A quantitative study

Karine Monier; L. Heliot; C. Rougeulle; E. Heard; M. Robert-Nicoud; C. Vourc'h; A. Bensimon; Y. Usson

doi:10.1159/000056869

Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: A quantitative study

Karine Monier
, L. Heliot
, C. Rougeulle
, E. Heard
, M. Robert-Nicoud
, C. Vourc'h
, A. Bensimon
, Y. Usson

Institut Albert Bonniot
Scripps Research Institute
Institut Pasteur, Paris
Unité de Génétique Moléculaire Murine
Laboratoire de Biophysique de l'ADN

Research output: Contribution to journal › Article › peer-review

Abstract

Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.

Original language	English
Pages (from-to)	59-62
Number of pages	4
Journal	Cytogenetics and Cell Genetics
Volume	92
Issue number	1-2
DOIs	https://doi.org/10.1159/000056869
Publication status	Published - 1 Jan 2001
Externally published	Yes

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abstract = "Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25\% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.",

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AU - Monier, Karine

AU - Heliot, L.

AU - Rougeulle, C.

AU - Heard, E.

AU - Robert-Nicoud, M.

AU - Vourc'h, C.

AU - Bensimon, A.

AU - Usson, Y.

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N2 - Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.

AB - Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.

U2 - 10.1159/000056869

DO - 10.1159/000056869

M3 - Article

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JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

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ER -

Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: A quantitative study

Abstract

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