In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues

Shensi Shen; Isabelle Girault; Hélène Malka-Mahieu; Caroline Robert; Stéphan Vagner

doi:10.1016/j.xpro.2021.100621

In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues

Shensi Shen
, Isabelle Girault
, Hélène Malka-Mahieu
, Caroline Robert
, Stéphan Vagner

Institute of Thoracic Oncology
Sichuan University
Gustave Roussy Comprehensive Cancer Institute
Université PSL
Université Paris-Saclay
Equipe labellisée Ligue contre le cancer
Sichuan University

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-based procedure allowing the direct in situ visualization of the eIF4F complex, as well as its absolute quantification per cell using adapted image analysis software. For complete details on the use and execution of this protocol, please refer to Boussemart et al. (2014).

Original language	English
Article number	100621
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100621
Publication status	Published - 17 Sept 2021
Externally published	Yes

Keywords

Cell culture
Cell-based Assays
Microscopy
Molecular Biology

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10.1016/j.xpro.2021.100621

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abstract = "The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-based procedure allowing the direct in situ visualization of the eIF4F complex, as well as its absolute quantification per cell using adapted image analysis software. For complete details on the use and execution of this protocol, please refer to Boussemart et al. (2014).",

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AU - Shen, Shensi

AU - Girault, Isabelle

AU - Malka-Mahieu, Hélène

AU - Robert, Caroline

AU - Vagner, Stéphan

PY - 2021/9/17

Y1 - 2021/9/17

N2 - The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-based procedure allowing the direct in situ visualization of the eIF4F complex, as well as its absolute quantification per cell using adapted image analysis software. For complete details on the use and execution of this protocol, please refer to Boussemart et al. (2014).

AB - The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-based procedure allowing the direct in situ visualization of the eIF4F complex, as well as its absolute quantification per cell using adapted image analysis software. For complete details on the use and execution of this protocol, please refer to Boussemart et al. (2014).

KW - Cell culture

KW - Cell-based Assays

KW - Microscopy

KW - Molecular Biology

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DO - 10.1016/j.xpro.2021.100621

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In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues

Abstract

Keywords

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