Interaction of human DNA topoisomerase I with G-quartet structures

Paola B. Arimondo; Jean François Riou; Jean Louis Mergny; Jamal Tazi; Jian Sheng Sun; Thérèse Garestier; Claude Hélène

doi:10.1093/nar/28.24.4832

Interaction of human DNA topoisomerase I with G-quartet structures

Paola B. Arimondo
, Jean François Riou
, Jean Louis Mergny
, Jamal Tazi
, Jian Sheng Sun
, Thérèse Garestier
, Claude Hélène

CNRS/Museum National d'Histoire Naturelle/IRD/UPMC
Centre de Recherche de Vitry-Alfortville
INSERM U869
IGMM Institut de Génétique Moléculaire de Montpellier

Research output: Contribution to journal › Article › peer-review

Abstract

Because of their role in the control of the topological state of DNA, topoisomerases are ubiquitous and vital enzymes, which participate in nearly all events related to DNA metabolism including replication and transcription. We show here that human topoisomerase I (Topo I) plays an unexpected role of 'molecular matchmaker' for G-quartet formation. G-quadruplexes are multi-stranded structures held together by square planes of four guanines ('G-quartets') interacting by forming Hoogsteen hydrogen bonds. Topo I is able to promote the formation of four-stranded intermolecular DNA structures when added to single-stranded DNA containing a stretch of at least five guanines. We provide evidence that these complexes are parallel G-quartet structures, mediated by tetrads of hydrogen-bonded guanine. In addition, Topo I binds specifically to pre-formed parallel and anti-parallel G4-DNA.

Original language	English
Pages (from-to)	4832-4838
Number of pages	7
Journal	Nucleic Acids Research
Volume	28
Issue number	24
DOIs	https://doi.org/10.1093/nar/28.24.4832
Publication status	Published - 15 Dec 2000
Externally published	Yes

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title = "Interaction of human DNA topoisomerase I with G-quartet structures",

abstract = "Because of their role in the control of the topological state of DNA, topoisomerases are ubiquitous and vital enzymes, which participate in nearly all events related to DNA metabolism including replication and transcription. We show here that human topoisomerase I (Topo I) plays an unexpected role of 'molecular matchmaker' for G-quartet formation. G-quadruplexes are multi-stranded structures held together by square planes of four guanines ('G-quartets') interacting by forming Hoogsteen hydrogen bonds. Topo I is able to promote the formation of four-stranded intermolecular DNA structures when added to single-stranded DNA containing a stretch of at least five guanines. We provide evidence that these complexes are parallel G-quartet structures, mediated by tetrads of hydrogen-bonded guanine. In addition, Topo I binds specifically to pre-formed parallel and anti-parallel G4-DNA.",

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doi = "10.1093/nar/28.24.4832",

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AU - Arimondo, Paola B.

AU - Riou, Jean François

AU - Mergny, Jean Louis

AU - Tazi, Jamal

AU - Sun, Jian Sheng

AU - Garestier, Thérèse

AU - Hélène, Claude

PY - 2000/12/15

Y1 - 2000/12/15

N2 - Because of their role in the control of the topological state of DNA, topoisomerases are ubiquitous and vital enzymes, which participate in nearly all events related to DNA metabolism including replication and transcription. We show here that human topoisomerase I (Topo I) plays an unexpected role of 'molecular matchmaker' for G-quartet formation. G-quadruplexes are multi-stranded structures held together by square planes of four guanines ('G-quartets') interacting by forming Hoogsteen hydrogen bonds. Topo I is able to promote the formation of four-stranded intermolecular DNA structures when added to single-stranded DNA containing a stretch of at least five guanines. We provide evidence that these complexes are parallel G-quartet structures, mediated by tetrads of hydrogen-bonded guanine. In addition, Topo I binds specifically to pre-formed parallel and anti-parallel G4-DNA.

AB - Because of their role in the control of the topological state of DNA, topoisomerases are ubiquitous and vital enzymes, which participate in nearly all events related to DNA metabolism including replication and transcription. We show here that human topoisomerase I (Topo I) plays an unexpected role of 'molecular matchmaker' for G-quartet formation. G-quadruplexes are multi-stranded structures held together by square planes of four guanines ('G-quartets') interacting by forming Hoogsteen hydrogen bonds. Topo I is able to promote the formation of four-stranded intermolecular DNA structures when added to single-stranded DNA containing a stretch of at least five guanines. We provide evidence that these complexes are parallel G-quartet structures, mediated by tetrads of hydrogen-bonded guanine. In addition, Topo I binds specifically to pre-formed parallel and anti-parallel G4-DNA.

UR - https://www.scopus.com/pages/publications/0034671853

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DO - 10.1093/nar/28.24.4832

M3 - Article

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JO - Nucleic Acids Research

JF - Nucleic Acids Research

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Interaction of human DNA topoisomerase I with G-quartet structures

Abstract

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