Interactions of cryptolepine and neocryptolepine with unsual DNA structures

  • Lionel Guittat
  • , Patrizia Alberti
  • , Frédéric Rosu
  • , Sabine Van Miert
  • , Emilie Thetiot
  • , Luc Pieters
  • , Valérie Gabelica
  • , Edwin De Pauw
  • , Alexandre Ottaviani
  • , Jean François Riou
  • , Jean Louis Mergny

Research output: Contribution to journalArticlepeer-review

Abstract

Cryptolepine, the main alkaloid present in the roots of Cryptolepis sanguinolenta, presents a large spectrum of biological properties. It has been reported to behave like a DNA intercalator with a preference for GC-rich sequences. In this study, dialysis competition assay and mass spectrometry experiments were used to determine the affinity of cryptolepine and neocryptolepine for DNA structures among duplexes, triplexes, quadruplexes and single strands. Our data confirm that cryptolepine and neocryptolepine prefer GC over AT-rich duplex sequences, but also recognize triplex and quadruplex structures. These compounds are weak telomerase inhibitors and exhibit a significant preference for triplexes over quadruplexes or duplexes.

Original languageEnglish
Pages (from-to)535-547
Number of pages13
JournalBiochimie
Volume85
Issue number5
DOIs
Publication statusPublished - 1 May 2003
Externally publishedYes

Keywords

  • Cryptolepine
  • Quadruplex
  • Telomerase
  • Telomere
  • Triplex

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