TY - JOUR
T1 - Intercalation of ethidium bromide into a triple-stranded oligonucleotide
AU - Mergny, Jean louis
AU - Collier, David
AU - Rougée, Michel
AU - Montenay-garestier, Thérèse
AU - Hélène, Claude
N1 - Funding Information:
We thank J.S. Sun for preliminary molecular modelling studies. Work of J.L. Mergny was supported by a financial grant from the 'Institut de Formation Supe'rieure Bio-M6dicale' and Rhone-Poulenc.
PY - 1991/4/11
Y1 - 1991/4/11
N2 - We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresponding duplex. Energy transfer from base triplets to ethidium shows that ethidium is intercalated into the triple helix. The spectroscopic characteristics of ethidium intercalated into a triplex are similar to those observed for Intercalation into duplex DNA.
AB - We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresponding duplex. Energy transfer from base triplets to ethidium shows that ethidium is intercalated into the triple helix. The spectroscopic characteristics of ethidium intercalated into a triplex are similar to those observed for Intercalation into duplex DNA.
U2 - 10.1093/nar/19.7.1521
DO - 10.1093/nar/19.7.1521
M3 - Article
C2 - 2027760
AN - SCOPUS:0025921984
SN - 0305-1048
VL - 19
SP - 1521
EP - 1526
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 7
ER -