TY - GEN
T1 - Ionic contrast terahertz near-field imaging of axonal activity and water fluxes
AU - Masson, Jean Baptiste
AU - Sauviat, Martin Pierre
AU - Martin, Jean Louis
AU - Gallot, Guilhem
PY - 2007/4/30
Y1 - 2007/4/30
N2 - We demonstrated the direct and noninvasive imaging of functional neurons,1 as well as auricular heart muscle electrical activity 2 by Ionic Contrast Terahertz (ICT) near-field microscopy. This technique provides quantitative measurements of ionic concentrations in both the intracellular and extracellular compartments and opens the way to direct noninvasive imaging of neurons during electrical, toxin, or thermal stresses. Furthermore, neuronal activity results from both a precise control of transient variations in ionic conductances and a much less studied water exchange between the extracellular matrix and the intraaxonal compartment. The developed ICT technique associated with a full three-dimensional simulation of the axon-aperture near-field system allows a precise measurement of the axon geometry and therefore the direct visualization of neuron swelling induced by temperature change or neurotoxin poisoning. This technique should then provide grounds for the development of advanced functional neuroimaging methods based on diffusion anisotropy of water molecules.
AB - We demonstrated the direct and noninvasive imaging of functional neurons,1 as well as auricular heart muscle electrical activity 2 by Ionic Contrast Terahertz (ICT) near-field microscopy. This technique provides quantitative measurements of ionic concentrations in both the intracellular and extracellular compartments and opens the way to direct noninvasive imaging of neurons during electrical, toxin, or thermal stresses. Furthermore, neuronal activity results from both a precise control of transient variations in ionic conductances and a much less studied water exchange between the extracellular matrix and the intraaxonal compartment. The developed ICT technique associated with a full three-dimensional simulation of the axon-aperture near-field system allows a precise measurement of the axon geometry and therefore the direct visualization of neuron swelling induced by temperature change or neurotoxin poisoning. This technique should then provide grounds for the development of advanced functional neuroimaging methods based on diffusion anisotropy of water molecules.
U2 - 10.1117/12.698309
DO - 10.1117/12.698309
M3 - Conference contribution
AN - SCOPUS:34247337334
SN - 0819465542
SN - 9780819465542
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Y2 - 22 January 2007 through 24 January 2007
ER -