TY - GEN
T1 - Label-free THG imaging of bone tissue microstructure
T2 - Advances in Microscopic Imaging II 2019
AU - Genthial, Rachel
AU - Beaurepaire, Emmanuel
AU - Schanne-Klein, Marie Claire
AU - Peyrin, Françoise
AU - Olivier, Cécile
AU - Gerbaix, Maude
AU - Vico, Laurence
AU - Gourrier, Aurélien
AU - Débarre, Delphine
N1 - Publisher Copyright:
© 2019 SPIE.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - In bone tissue, multiscale interfaces provide the structural basis of essential bone functions and contribute to its macroscopic mechanical properties. The lacuno-canalicular network (LCN) hosting the osteocytes in the bone matrix, in particular, represents a biological signature of the mechanotransduction activity in response to external biomechanical loading. We have demonstrated that label-free third-harmonic generation (THG) microscopy reveals the structure of the LCN in 3D with submicron precision over millimetric fields of view compatible with histology and can be coupled to second-harmonic generation (SHG) signals relating to the collagen organization in the bone matrix. Taking advantage of these label-free imaging methods, we investigate the impact of microgravity on the LCN structure in mice following a 1-month space flight. We show that our current lack of understanding of the extent of the LCN heterogeneity at the organ level hinders the interpretation of such investigations based on a limited number of samples and we discuss the implications for future biomedical studies.
AB - In bone tissue, multiscale interfaces provide the structural basis of essential bone functions and contribute to its macroscopic mechanical properties. The lacuno-canalicular network (LCN) hosting the osteocytes in the bone matrix, in particular, represents a biological signature of the mechanotransduction activity in response to external biomechanical loading. We have demonstrated that label-free third-harmonic generation (THG) microscopy reveals the structure of the LCN in 3D with submicron precision over millimetric fields of view compatible with histology and can be coupled to second-harmonic generation (SHG) signals relating to the collagen organization in the bone matrix. Taking advantage of these label-free imaging methods, we investigate the impact of microgravity on the LCN structure in mice following a 1-month space flight. We show that our current lack of understanding of the extent of the LCN heterogeneity at the organ level hinders the interpretation of such investigations based on a limited number of samples and we discuss the implications for future biomedical studies.
KW - Bone tissue microstructure
KW - Label-free imaging
KW - Second-harmonic generation microscopy
KW - Third-harmonic generation microscopy
U2 - 10.1117/12.2527029
DO - 10.1117/12.2527029
M3 - Conference contribution
AN - SCOPUS:85074292399
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Advances in Microscopic Imaging II
A2 - Beaurepaire, Emmanuel
A2 - Pavone, Francesco Saverio
PB - SPIE
Y2 - 26 June 2019 through 27 June 2019
ER -