Large-scale live imaging of adult neural stem cells in their endogenous niche

Nicolas Dray; Sébastien Bedu; Nelly Vuillemin; Alessandro Alunni; Marion Coolen; Monika Krecsmarik; Willy Supatto; Emmanuel Beaurepaire; Laure Bally-Cuif

doi:10.1242/dev.123018

Large-scale live imaging of adult neural stem cells in their endogenous niche

Nicolas Dray
, Sébastien Bedu
, Nelly Vuillemin
, Alessandro Alunni
, Marion Coolen
, Monika Krecsmarik
, Willy Supatto
, Emmanuel Beaurepaire
, Laure Bally-Cuif

Research output: Contribution to journal › Article › peer-review

Abstract

Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.

Original language	English
Pages (from-to)	3592-3600
Number of pages	9
Journal	Development
Volume	142
Issue number	20
DOIs	https://doi.org/10.1242/dev.123018
Publication status	Published - 15 Oct 2015

Keywords

Live imaging
Multiphoton microscopy
Neural stem cell
Pallium
SHG/THG
Zebrafish

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10.1242/dev.123018

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title = "Large-scale live imaging of adult neural stem cells in their endogenous niche",

abstract = "Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.",

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AU - Dray, Nicolas

AU - Bedu, Sébastien

AU - Vuillemin, Nelly

AU - Alunni, Alessandro

AU - Coolen, Marion

AU - Krecsmarik, Monika

AU - Supatto, Willy

AU - Beaurepaire, Emmanuel

AU - Bally-Cuif, Laure

PY - 2015/10/15

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AB - Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.

KW - Live imaging

KW - Multiphoton microscopy

KW - Neural stem cell

KW - Pallium

KW - SHG/THG

KW - Zebrafish

UR - https://www.scopus.com/pages/publications/84944790606

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VL - 142

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EP - 3600

JO - Development

JF - Development

IS - 20

ER -

Large-scale live imaging of adult neural stem cells in their endogenous niche

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