@article{a9c5e3a5a5ff4e89a4deeb0e5170b155,
title = "Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders",
abstract = "We report here the details of G4-FID (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex-DNA binding affinity and quadruplex- over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex- and duplex-DNA matrices. The original protocol was tested using various quadruplex- and duplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metallo-organic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays.",
keywords = "Fluorescent intercalator displacement, G-quadruplex DNA, G-quadruplex ligands, Thiazole orange",
author = "D. Monchaud and C. Allain and H. Bertrand and N. Smargiasso and F. Rosu and V. Gabelica and \{De Cian\}, A. and Mergny, \{J. L.\} and Teulade-Fichou, \{M. P.\}",
note = "Funding Information: We would like to deeply thank our daily collaborators for their synthetic work (in particular Candide Hounsou, Elsa De Lemos), and Dr. Sophie Bombard for her scientific support. The Centre National de la Recherche Scientifique (CNRS) and Commissariat {\`a} l'Energie Atomique (CEA) are also gratefully acknowledged for their financial support (C.A., H.B.). Financial support from the Fonds de la Recherche Scientifique-FNRS (Belgium) is also acknowledged (FRFC grant 2.4.623.05, FRIA doctoral fellowship to N.S., post-doctoral fellowship to F.R., and research associate position to V.G.). M.P.T.F. and V.G. also thank the “Tournesol program (CGRI-FNRS-CNRS) for bilateral France-Belgium collaborations”. J.-L.M. and A.D.C. thank the European FP6 “MolCancerMed” program (LSHC-CT-2004-502943) for financial support. ",
year = "2008",
month = aug,
day = "1",
doi = "10.1016/j.biochi.2008.02.019",
language = "English",
volume = "90",
pages = "1207--1223",
journal = "Biochimie",
issn = "0300-9084",
number = "8",
}