TY - GEN
T1 - Light emission properties and biological applications of lanthanide doped oxide nanoparticles
AU - Mialon, Genevieve
AU - Giaume, Domitille
AU - Moreau, Melanie
AU - Casanova, Didier
AU - Nguyen, Thanh Liem
AU - Popoff, Michel Robert
AU - Alexandrou, Antigoni
AU - Gacoin, Thierry
AU - Boilot, Jean Pierre
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Yttrium vanadate particles doped with europium are studied for their applications as biomolecule labels. Two parts of our recent work will be presented. The first concerns the thermal treatment of particles incorporated in a silica matrix. After annealing at 1000°C and redispersion in water by dissolution of the silica matrix, the structural and optical properties are greatly improved : without any modification of size, the obtained nanoparticles appear as perfect single crystals of 33 nm and have the same emission properties as the bulk material. Their quantum yield is 37% for 10% europium doping and the emission lifetime is 0.8 ms. The second aspect concerns the functionalization of the nanoparticles by the sol-gel condensation of aminopropyltriethoxysilane at the surface of particles. The chemical accessibility of amino groups grafted on the particles is measured by reaction with an organic fluorescent tag FITC (fluorescein isothiocyanate). These amine-coated nanoparticles were coupled to a peptidic toxin via a cross-linker and allowed observation of the toxin motion bound to its membrane receptor.
AB - Yttrium vanadate particles doped with europium are studied for their applications as biomolecule labels. Two parts of our recent work will be presented. The first concerns the thermal treatment of particles incorporated in a silica matrix. After annealing at 1000°C and redispersion in water by dissolution of the silica matrix, the structural and optical properties are greatly improved : without any modification of size, the obtained nanoparticles appear as perfect single crystals of 33 nm and have the same emission properties as the bulk material. Their quantum yield is 37% for 10% europium doping and the emission lifetime is 0.8 ms. The second aspect concerns the functionalization of the nanoparticles by the sol-gel condensation of aminopropyltriethoxysilane at the surface of particles. The chemical accessibility of amino groups grafted on the particles is measured by reaction with an organic fluorescent tag FITC (fluorescein isothiocyanate). These amine-coated nanoparticles were coupled to a peptidic toxin via a cross-linker and allowed observation of the toxin motion bound to its membrane receptor.
M3 - Conference contribution
AN - SCOPUS:70350327753
SN - 9781605608389
T3 - Materials Research Society Symposium Proceedings
SP - 1
EP - 7
BT - Quantum-Dot and Nanoparticle Bioconjugates-Tools for Sensing and Biomedical Imaging
T2 - 2007 MRS Fall Meeting
Y2 - 26 November 2007 through 30 November 2007
ER -