Low-coherence in depth microscopy for biological tissues imaging: Design of a real time control system

L. Blanchot; M. Lebec; E. Beaurepaire; P. Gleyzes; A. C. Boccara; H. Saint-Jalmes

doi:10.1117/12.301057

Low-coherence in depth microscopy for biological tissues imaging: Design of a real time control system

L. Blanchot
, M. Lebec
, E. Beaurepaire
, P. Gleyzes
, A. C. Boccara
, H. Saint-Jalmes

Université de PARIS XII

Research output: Contribution to journal › Conference article › peer-review

Abstract

We describe the design of a versatile electronic system performing a lock-in detection in parallel on every pixel of a 2D CCD camera. The system is based on a multiplexed lock-in detection method that requires accurate synchronisation of the camera, the excitation signal and the processing computer. This device has been incorporated in an imaging setup based on the optical coherence tomography (OCT) principle, enabling to acquire a full 2D head-on image without scanning. The imaging experiment is implemented on a modified commercial microscope. Lateral resolution is on the order of 2 μm, and the coherence length of the light source (LED) defines an axial resolution of approximately 8 μm. Images of onion cells a few hundred microns deep into the sample are obtained with 100 dB sensitivity.

Original language	English
Pages (from-to)	198-204
Number of pages	7
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3194
DOIs	https://doi.org/10.1117/12.301057
Publication status	Published - 1 Dec 1997
Externally published	Yes
Event	Proceedings of Photon Propagation in Tissues III - San Remo, Italy Duration: 6 Sept 1997 → 8 Sept 1997

Keywords

CCD camera
Hardware design
Interferometry
Microscopy
Multiplexed lock-in detection
OCT

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10.1117/12.301057

Cite this

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title = "Low-coherence in depth microscopy for biological tissues imaging: Design of a real time control system",

abstract = "We describe the design of a versatile electronic system performing a lock-in detection in parallel on every pixel of a 2D CCD camera. The system is based on a multiplexed lock-in detection method that requires accurate synchronisation of the camera, the excitation signal and the processing computer. This device has been incorporated in an imaging setup based on the optical coherence tomography (OCT) principle, enabling to acquire a full 2D head-on image without scanning. The imaging experiment is implemented on a modified commercial microscope. Lateral resolution is on the order of 2 μm, and the coherence length of the light source (LED) defines an axial resolution of approximately 8 μm. Images of onion cells a few hundred microns deep into the sample are obtained with 100 dB sensitivity.",

keywords = "CCD camera, Hardware design, Interferometry, Microscopy, Multiplexed lock-in detection, OCT",

author = "L. Blanchot and M. Lebec and E. Beaurepaire and P. Gleyzes and Boccara, \{A. C.\} and H. Saint-Jalmes",

year = "1997",

month = dec,

day = "1",

doi = "10.1117/12.301057",

language = "English",

volume = "3194",

pages = "198--204",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

note = "Proceedings of Photon Propagation in Tissues III ; Conference date: 06-09-1997 Through 08-09-1997",

}

TY - JOUR

T1 - Low-coherence in depth microscopy for biological tissues imaging

T2 - Proceedings of Photon Propagation in Tissues III

AU - Blanchot, L.

AU - Lebec, M.

AU - Beaurepaire, E.

AU - Gleyzes, P.

AU - Boccara, A. C.

AU - Saint-Jalmes, H.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - We describe the design of a versatile electronic system performing a lock-in detection in parallel on every pixel of a 2D CCD camera. The system is based on a multiplexed lock-in detection method that requires accurate synchronisation of the camera, the excitation signal and the processing computer. This device has been incorporated in an imaging setup based on the optical coherence tomography (OCT) principle, enabling to acquire a full 2D head-on image without scanning. The imaging experiment is implemented on a modified commercial microscope. Lateral resolution is on the order of 2 μm, and the coherence length of the light source (LED) defines an axial resolution of approximately 8 μm. Images of onion cells a few hundred microns deep into the sample are obtained with 100 dB sensitivity.

AB - We describe the design of a versatile electronic system performing a lock-in detection in parallel on every pixel of a 2D CCD camera. The system is based on a multiplexed lock-in detection method that requires accurate synchronisation of the camera, the excitation signal and the processing computer. This device has been incorporated in an imaging setup based on the optical coherence tomography (OCT) principle, enabling to acquire a full 2D head-on image without scanning. The imaging experiment is implemented on a modified commercial microscope. Lateral resolution is on the order of 2 μm, and the coherence length of the light source (LED) defines an axial resolution of approximately 8 μm. Images of onion cells a few hundred microns deep into the sample are obtained with 100 dB sensitivity.

KW - CCD camera

KW - Hardware design

KW - Interferometry

KW - Microscopy

KW - Multiplexed lock-in detection

KW - OCT

U2 - 10.1117/12.301057

DO - 10.1117/12.301057

M3 - Conference article

AN - SCOPUS:0031289797

SN - 0277-786X

VL - 3194

SP - 198

EP - 204

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

Y2 - 6 September 1997 through 8 September 1997

ER -

Low-coherence in depth microscopy for biological tissues imaging: Design of a real time control system

Abstract

Keywords

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