Microinstrument for optical monitoring of endothelial cell migration under controlled tension/compression via integrated magnetic composite polymer actuation

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Abstract

We present a microfabricated platform that allows simultaneous application of controlled stretch/compression forces and fluid flow shear stresses during endothelial cell (EC) live-cell monitoring. Our device employs a highly flexible magnetic composite polymer (M-CP) for actuation of a flexible microchannel system. We combine our M-CP with micropatterned non-magnetic polydimethylsiloxane (PDMS), resulting in flexible microsystems with integrated actuators and microfluidic channels whereby we can optically visualize cells in order to monitor various aspects of cell behavior including migration, proliferation, and morphological changes. The M-CP can be rendered permanently magnetic, so it can be employed for both substrate tension and compression using the same electro-or permanent magnet with pole reversal. We have demonstrated proof-of-concept of an instrument designed to simultaneously stimulate ECs grown in microfluidic channels with both fluid flow and mechanical stretch/compression using the new M-CP actuators.

Original languageEnglish
Title of host publicationProceedings of the IEEE Conference on Nanotechnology
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages986-990
Number of pages5
ISBN (Electronic)9781479956227
DOIs
Publication statusPublished - 26 Nov 2014
Event2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 - Toronto, Canada
Duration: 18 Aug 201421 Aug 2014

Publication series

NameProceedings of the IEEE Conference on Nanotechnology
ISSN (Electronic)1944-9399

Conference

Conference2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
Country/TerritoryCanada
CityToronto
Period18/08/1421/08/14

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