Modular microinstrumentation for endothelial cell research

Bonnie L. Gray; Abdul I. Barakat; Deborah K. Lieu; Scott D. Collins; Rosemary L. Smith

Modular microinstrumentation for endothelial cell research

Bonnie L. Gray
, Abdul I. Barakat
, Deborah K. Lieu
, Scott D. Collins
, Rosemary L. Smith

University of California, Davis

Research output: Contribution to journal › Conference article › peer-review

Abstract

Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30-300 μm wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off-chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m², cuboidal cells grown in 200 μm wide microchannels progressively align and elongate in the direction of flow.

Original language	English
Pages (from-to)	88-94
Number of pages	7
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3912
Publication status	Published - 1 Jan 2000
Externally published	Yes
Event	Micro- and Nanotechnology for Biomedical and Environmental Applications - San Jose, CA, USA Duration: 26 Jan 2000 → 27 Jan 2000

Cite this

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title = "Modular microinstrumentation for endothelial cell research",

abstract = "Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30-300 μm wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off-chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m2, cuboidal cells grown in 200 μm wide microchannels progressively align and elongate in the direction of flow.",

author = "Gray, \{Bonnie L.\} and Barakat, \{Abdul I.\} and Lieu, \{Deborah K.\} and Collins, \{Scott D.\} and Smith, \{Rosemary L.\}",

year = "2000",

month = jan,

day = "1",

language = "English",

volume = "3912",

pages = "88--94",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

note = "Micro- and Nanotechnology for Biomedical and Environmental Applications ; Conference date: 26-01-2000 Through 27-01-2000",

}

TY - JOUR

T1 - Modular microinstrumentation for endothelial cell research

AU - Gray, Bonnie L.

AU - Barakat, Abdul I.

AU - Lieu, Deborah K.

AU - Collins, Scott D.

AU - Smith, Rosemary L.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30-300 μm wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off-chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m2, cuboidal cells grown in 200 μm wide microchannels progressively align and elongate in the direction of flow.

AB - Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30-300 μm wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off-chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m2, cuboidal cells grown in 200 μm wide microchannels progressively align and elongate in the direction of flow.

M3 - Conference article

AN - SCOPUS:0033879669

SN - 0277-786X

VL - 3912

SP - 88

EP - 94

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Micro- and Nanotechnology for Biomedical and Environmental Applications

Y2 - 26 January 2000 through 27 January 2000

ER -

Modular microinstrumentation for endothelial cell research

Abstract

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