Abstract
We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.
| Original language | English |
|---|---|
| Pages (from-to) | 815-818 |
| Number of pages | 4 |
| Journal | Nature Methods |
| Volume | 9 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 1 Aug 2012 |