Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging

Vincent Maioli; Antoine Boniface; Pierre Mahou; Júlia Ferrer Ortas; Lamiae Abdeladim; Emmanuel Beaurepaire; Willy Supatto

doi:10.1364/FIO.2020.FW5E.5

Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging

Vincent Maioli
, Antoine Boniface
, Pierre Mahou
, Júlia Ferrer Ortas
, Lamiae Abdeladim
, Emmanuel Beaurepaire
, Willy Supatto

Institut Polytechnique de Paris

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Light-sheet illumination improves multiphoton imaging speed. By optimizing laser pulse frequency using the quantification of photodamage in embryos, we demonstrate an order-of-magnitude signal increase in live two-photon light-sheet imaging.

Original language	English
Title of host publication	Frontiers in Optics - Proceedings Frontiers in Optics / Laser Science, Part of Frontiers in Optics + Laser Science APS/DLS, FiO 2020
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Electronic)	9781943580804
DOIs	https://doi.org/10.1364/FIO.2020.FW5E.5
Publication status	Published - 14 Sept 2020
Event	2020 Frontiers in Optics Conference, FiO 2020 - Washington, United States Duration: 14 Sept 2020 → 17 Sept 2020

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Conference

Conference	2020 Frontiers in Optics Conference, FiO 2020
Country/Territory	United States
City	Washington
Period	14/09/20 → 17/09/20

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10.1364/FIO.2020.FW5E.5

Cite this

Maioli, V., Boniface, A., Mahou, P., Ortas, J. F., Abdeladim, L., Beaurepaire, E., & Supatto, W. (2020). Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging. In Frontiers in Optics - Proceedings Frontiers in Optics / Laser Science, Part of Frontiers in Optics + Laser Science APS/DLS, FiO 2020 Article FW5E.5 (Optics InfoBase Conference Papers). Optica Publishing Group (formerly OSA). https://doi.org/10.1364/FIO.2020.FW5E.5

@inproceedings{fcf4be0e57124f158c713a5fae7cdc5f,

title = "Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging",

abstract = "Light-sheet illumination improves multiphoton imaging speed. By optimizing laser pulse frequency using the quantification of photodamage in embryos, we demonstrate an order-of-magnitude signal increase in live two-photon light-sheet imaging.",

author = "Vincent Maioli and Antoine Boniface and Pierre Mahou and Ortas, \{J{\'u}lia Ferrer\} and Lamiae Abdeladim and Emmanuel Beaurepaire and Willy Supatto",

note = "Publisher Copyright: {\textcopyright} OSA 2020 {\textcopyright} 2020 The Author(s); 2020 Frontiers in Optics Conference, FiO 2020 ; Conference date: 14-09-2020 Through 17-09-2020",

year = "2020",

month = sep,

day = "14",

doi = "10.1364/FIO.2020.FW5E.5",

language = "English",

series = "Optics InfoBase Conference Papers",

publisher = "Optica Publishing Group (formerly OSA)",

booktitle = "Frontiers in Optics - Proceedings Frontiers in Optics / Laser Science, Part of Frontiers in Optics + Laser Science APS/DLS, FiO 2020",

}

Maioli, V, Boniface, A, Mahou, P, Ortas, JF, Abdeladim, L, Beaurepaire, E & Supatto, W 2020, Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging. in Frontiers in Optics - Proceedings Frontiers in Optics / Laser Science, Part of Frontiers in Optics + Laser Science APS/DLS, FiO 2020., FW5E.5, Optics InfoBase Conference Papers, Optica Publishing Group (formerly OSA), 2020 Frontiers in Optics Conference, FiO 2020, Washington, United States, 14/09/20. https://doi.org/10.1364/FIO.2020.FW5E.5

Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging. / Maioli, Vincent; Boniface, Antoine; Mahou, Pierre et al.
Frontiers in Optics - Proceedings Frontiers in Optics / Laser Science, Part of Frontiers in Optics + Laser Science APS/DLS, FiO 2020. Optica Publishing Group (formerly OSA), 2020. FW5E.5 (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging

AU - Maioli, Vincent

AU - Boniface, Antoine

AU - Mahou, Pierre

AU - Ortas, Júlia Ferrer

AU - Abdeladim, Lamiae

AU - Beaurepaire, Emmanuel

AU - Supatto, Willy

PY - 2020/9/14

Y1 - 2020/9/14

N2 - Light-sheet illumination improves multiphoton imaging speed. By optimizing laser pulse frequency using the quantification of photodamage in embryos, we demonstrate an order-of-magnitude signal increase in live two-photon light-sheet imaging.

AB - Light-sheet illumination improves multiphoton imaging speed. By optimizing laser pulse frequency using the quantification of photodamage in embryos, we demonstrate an order-of-magnitude signal increase in live two-photon light-sheet imaging.

U2 - 10.1364/FIO.2020.FW5E.5

DO - 10.1364/FIO.2020.FW5E.5

M3 - Conference contribution

AN - SCOPUS:85106020967

T3 - Optics InfoBase Conference Papers

BT - Frontiers in Optics - Proceedings Frontiers in Optics / Laser Science, Part of Frontiers in Optics + Laser Science APS/DLS, FiO 2020

PB - Optica Publishing Group (formerly OSA)

T2 - 2020 Frontiers in Optics Conference, FiO 2020

Y2 - 14 September 2020 through 17 September 2020

ER -

Maioli V, Boniface A, Mahou P, Ortas JF, Abdeladim L, Beaurepaire E et al. Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging. In Frontiers in Optics - Proceedings Frontiers in Optics / Laser Science, Part of Frontiers in Optics + Laser Science APS/DLS, FiO 2020. Optica Publishing Group (formerly OSA). 2020. FW5E.5. (Optics InfoBase Conference Papers). doi: 10.1364/FIO.2020.FW5E.5

Multiphoton light-sheet microscopy at optimal pulse frequency for fast in vivo imaging

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