@inproceedings{43ecedcb5c2b43b997c464b605af75f0,
title = "Multiphoton light-sheet microscopy using wavelength mixing: Fast multicolor imaging of the beating Zebrafish heart with low photobleaching",
abstract = "Two-photon laser scanning microscopy has become a standard to map thick and live tissues. However, its application for fast and multicolor imaging remains challenging. To address this issue, we report on the implementation of mixed wavelength excitation in a two-photon light-sheet microscope. We illustrate the potential of the technique by recording sustained multicolor two-photon movies of the beating heart in zebrafish embryos with negligible photobleaching at 28 million pixels/second. In particular, 3D reconstructions of the heart periodic motion are obtained with sufficient spatiotemporal resolution to track the fast movements of individual cells during a cardiac cycle.",
keywords = "Two-photon microscopy, cardiovascular imaging, light-sheet microscopy, wavelength mixing",
author = "Pierre Mahou and Julien Vermot and Emmanuel Beaurepaire and Willy Supatto",
note = "Publisher Copyright: {\textcopyright} 2015 SPIE.; Multiphoton Microscopy in the Biomedical Sciences XV ; Conference date: 08-02-2015 Through 10-02-2015",
year = "2015",
month = jan,
day = "1",
doi = "10.1117/12.2079176",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Karsten Konig and So, \{Peter T. C.\} and Ammasi Periasamy",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XV",
}